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16321929[PMID] - PMC - NCBI

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1.
FIG. 6.

FIG. 6. From: Distribution and Expression of the ZmpA Metalloprotease in the Burkholderia cepacia Complex.

Complementation of zmpA protease-deficient strains with Pc715j zmpA in trans. The assay shown is representative of two experiments performed in triplicate. Only strains J415 and C5424 had extracellular proteolytic activity when harboring pSG200. Strain CEP 509 produces a dark melanin-like pigment, which is visible around the colonies, but no zone of clearing was detectable.

S. Gingues, et al. J Bacteriol. 2005 Dec;187(24):8247-8255.
2.
FIG. 3.

FIG. 3. From: Distribution and Expression of the ZmpA Metalloprotease in the Burkholderia cepacia Complex.

Detection of ZmpA in culture supernatants. Western immunoblot of trichloroacetic acid-precipitated supernatants reacted with anti-ZmpA antibody. Lane 1, Pc715j; lane 2, K56-2; lane 3, Cep 511; lane 4, ATCC 17765; lane 5, LMG 14086; lane 6, LMG 19182; lane 7, Cep 509; lane 8, J2315; lane 9, J415; lane 10, C5424; lane 11, LMG 14294; lane 12, ZmpA.

S. Gingues, et al. J Bacteriol. 2005 Dec;187(24):8247-8255.
3.
FIG. 4.

FIG. 4. From: Distribution and Expression of the ZmpA Metalloprotease in the Burkholderia cepacia Complex.

Comparison of zmpA::lacZ expression in zmpA protease-proficient and zmpA protease-deficient strains. Expression of the zmpA::lacZ fusion contained on pSG208 was determined by measuring β-galactosidase assays. Assays were conducted in triplicate and repeated at least twice. Values shown are the mean percentages of K56-2 activity, which is represented as 100%, and are of a representative experiment. β-Galactosidase activity resulting from strains with the pUCP28T vector control was negligible and is not shown.

S. Gingues, et al. J Bacteriol. 2005 Dec;187(24):8247-8255.
4.
FIG. 5.

FIG. 5. From: Distribution and Expression of the ZmpA Metalloprotease in the Burkholderia cepacia Complex.

Comparison of zmpA expression in zmpA protease-proficient and zmpA protease-deficient strains by quantitative real-time RT-PCR. Expression of zmpA and ndh were determined by absolute quantification. Concentrations of the mRNA level of each strain are expressed as moles of target gene mRNA per mole compared to the concentration of the standard curve. The bars represent the means ± standard deviation of triplicate assays. Black bars, zmpA; gray bars, ndh.

S. Gingues, et al. J Bacteriol. 2005 Dec;187(24):8247-8255.
5.
FIG. 2.

FIG. 2. From: Distribution and Expression of the ZmpA Metalloprotease in the Burkholderia cepacia Complex.

Detection of zmpA in the B. cepacia complex by Southern hybridization. Southern blot of genomic DNA from representative strains digested with PstI, hybridized with a 250-bp fragment internal to zmpA, amplified by PCR, and labeled with 32P. Lane 1, ATCC 25416; lane 2, C5393; lane 3, LMG 13010; lane 4, J2315; lane 5, K56-2; lane 6, LMG 14294; lane 7, LMG 14086; lane 8, PC259; lane 9, LMG 16232; lane 10, LMG 18943; lane 11, LMG 19468T; lane 12, LMG 19467; lane 13, LMG 17828; lane 14, LMG 20980; lane 15, LMG 20982; lane 16, LMG 21822; lane 17, Pc715j.

S. Gingues, et al. J Bacteriol. 2005 Dec;187(24):8247-8255.
6.
FIG. 1.

FIG. 1. From: Distribution and Expression of the ZmpA Metalloprotease in the Burkholderia cepacia Complex.

Detection of zmpA in the B. cepacia complex by PCR. Lanes: 1, 1-kb Plus DNA ladder (Invitrogen); 2, ATCC 25416T; 3, ATCC 17759; 4, CEP 509; 5, LMG 17997; 6, C5393; 7, LMG 13010; 8, C1576; 9, CF-A1-1; 10, JTC; 11, C1962; 12, ATCC 17616; 13, 249-2; 14, J2315; 15, Pc715j; 16, K56-2; 17, C5424; 18, C6433; 19, C1394; 20, 1-kb Plus DNA ladder (Invitrogen); 21, 1-kb Plus DNA ladder (Invitrogen); 22, PC184; 23, CEP 511; 24, J415; 25, ATCC 17765; 26, LMG 14294; 27, C7322; 28, LMG 14086; 29, LMG 18888; 30, PC259; 31, LMG 16232; 32, FC441; 33, LMG 10929T; 34, L06; 35, LMG 18943; 36, LMG 17828; 37, LMG 19182T; 38, LMG 19467; 39, H2O control; 40, 1-kb Plus DNA ladder (Invitrogen); 41, 1-kb Plus DNA ladder (Invitrogen); 42, LMG 19468T; 43, LMG 20980T; 44, LMG 20983; 45, LMG 16670; 46, LMG 20982; 47, LMG 21822; 48, LMG 14191T; and 49, H2O control. B. viet., B. vietnamiensis; B. dol., B. dolosa; B. ambif., B. ambifaria; B. anth., B. anthina; and B. pyrr., B. pyrrocinia.

S. Gingues, et al. J Bacteriol. 2005 Dec;187(24):8247-8255.

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