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PubMed Central, Fig. 2. : Microbiology. 2011 Nov; 157(Pt 11): 3195–3202. doi:  10.1099/mic.0.052498-0
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Fig. 2.

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Initial attachment of T. forsythia to glycoprotein-coated surfaces. (a) Adhesion to glycoproteins was assessed for the wild-type strain in the absence (black bars) or presence of oseltamivir (grey bars). Biofilms were set up in triplicate and wells were supplemented with 6 mM sialic acid (sialic), or were coated with 6 mM mucin, fetuin and asialofetuin (as-fet), 2 µg human serum ml−1 (serum) or saliva, with and without the addition of 10 mM oseltamivir to the TSB medium, as indicated at the time of inoculation. Glycoproteins were coated on the 96-well plate overnight at 4 °C and washed before inoculation with T. forsythia to a final OD600 of 0.05. Data are the mean number of cells with sds after harvesting from wells after 3 h. Differences between mean values in this experiment were evaluated by t test, with P<0.05 being taken as the level of significance (*statistically significant). (b) Adhesion to glycoproteins was compared as above between the wild-type (wt) strain and ΔnanH mutant strain of T. forsythia (ΔnanH).

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