University at Buffalo - The State University of New York
Skip to Content
Structure of the LPS O-chain from Fusobacterium nucleatum strain 10953, containing sialic acid. - PubMed - NCBI
Format

Send to

Choose Destination
See comment in PubMed Commons below
Carbohydr Res. 2017 Feb 22;440-441:38-42. doi: 10.1016/j.carres.2017.01.009. Epub 2017 Jan 28.

Structure of the LPS O-chain from Fusobacterium nucleatum strain 10953, containing sialic acid.

Author information

1
Vaccine Program, Human Health Therapeutics Portfolio, National Research Council, Ottawa, ON, K1A 0R6, Canada. Electronic address: evguenii.vinogradov@nrc-cnrc.gc.ca.
2
Vaccine Program, Human Health Therapeutics Portfolio, National Research Council, Ottawa, ON, K1A 0R6, Canada.
3
Department of Oral Biology, University at Buffalo, Buffalo, NY, 14214, USA.

Abstract

Fusobacterium nucleatum is an anaerobic bacterium found in the human mouth where it causes periodontitis. Recently, it has been gaining attention as a potential causative agent for colorectal cancer and is strongly linked with pregnancy complications including pre-term and still births. Little is known about virulence factors of this organism and thus we have initiated studies to examine the bacterial surface glycochemistry. Consistent with a recent paper suggesting that F. nucleatum strain 10593 can synthesize sialic acid, a staining technique identified sialic acid on the bacterial surface. We isolated lipopolysaccharide from this F. nucleatum strain and performed structural analysis on the O-antigen. Our studies identified a trisaccharide repeating unit of the O-antigen with the following structure: -[→4)-α-Neup5Ac-(2 → 4)-β-d-Galp-(1 → 3)-α-d-FucpNAc4NAc-(1-]- where Ac indicates 4-N-acetylation of ∼30% FucNAc4N residues. The presence of sialic acid as a constituent of the O-antigen is consistent with recent data identifying de novo sialic acid synthesis in this strain.

KEYWORDS:

Fusobacterium nucleatum; Lipopolysaccharide; Sialic acid

PMID:
28199859
PMCID:
PMC5502818
[Available on 2018-02-22]
DOI:
10.1016/j.carres.2017.01.009
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center