(a) RAW264.7 macrophages were transduced with Ad5-GFP or Ad5-TTP and stimulated with Aggregatibacter actinomycetemcomitans lipopolysaccharide (LPS) (1 µg/ml). RNA was isolated 18 hours after stimulation and subjected to real-time reverse transcription-PCR measurement of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and cyclooxygenase-2 (COX-2) normalized to glyceraldehyde 3-phosphate dehydrogenase mRNA (n = 3 each). (b) HeLa cells were “adenofected” with luciferase reporter constructs and Ad5-TTP [75 multiplicity of infection (MOI)], Ad5-GFP (75 MOI), or no adenovirus (Ad). Luciferase reporters contained 3′-untranslated region (3′-UTR) of TNF-α, IL-6, and COX-2, or non-ARE control pGL3-control. Luciferase activity was measured and normalized to total protein (n = 3; *P < 0.05, **P < 0.01, ***P < 0.001). TTP, tristetraprolin.