University at Buffalo - The State University of New York
Skip to Content
Sialic acid, periodontal pathogens and Tannerella forsythia: stick around and enjoy the feast!
PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

Logo of wtpaEurope PMCEurope PMC Funders GroupSubmit a Manuscript
 
Mol Oral Microbiol. Author manuscript; available in PMC 2014 June 9.
Published in final edited form as:
PMCID: PMC4049603
EMSID: EMS58973

Sialic acid, periodontal pathogens and Tannerella forsythia: stick around and enjoy the feast!

Abstract

Periodontal pathogens, like any other human commensal or pathogenic bacterium, must possess both the ability to acquire the necessary growth factors but also the means to adhere to surfaces or reside and survive in their environmental niche. Recent evidence has suggested that sialic acid containing host molecules may provide both of these requirements in vivo for several periodontal pathogens but most notably for the red complex organism Tannerella forsythia. Several other periodontal pathogens also possess sialic acid scavenging enzymes – sialidases, which can also expose adhesive epitopes, but might also act as adhesins in their own right. In addition recent experimental work coupled with the release of several genome sequences has revealed that periodontal bacteria have a range of sialic acid uptake and utilisation systems while others may also use sialic acid as a cloaking device on their surface to mimic host and avoid immune recognition. This review will focus on these systems in a range of periodontal bacteria with a focus on T. forsythia.

Sialic acid as a growth factor or carbon source

An increasing number of human pathogens are being uncovered that have the ability to use sialic acid as a growth factor or sole carbon source (Vimr ref, Severi ref, others). These now include representatives of several bacterial genera (including Neisseria (ref), Haemophilus (ref), Bacteroides (ref), Fusobacteria (ref) and Streptococci ref) that inhabit a range of biological niches within the human body from the oral cavity through the respiratory system and into the gastrointestinal and urinary tracts, although none have been found in free-living bacterial species to date.

While sialic acid seems an obvious source of carbon for bacterial pathogens and other human and mammalian dwelling bacteria since it is present on the surface of glycoproteins, gangliosides and sphingolipids (ref), its role in the biology and pathogenesis of periodontal pathogens is only now coming to light. The discovery that the nutritionally fastidious periodontal pathogen Tannerella forsythia is able to substitute its requirement for N-acetyl muramic acid (MurNAc) (Wyss), a building block used to produce cell wall peptidoglycans, with sialic acid in biofilm culture (ref) was not only surprising but also suggested that in vivo it may actually be more adaptable than its fastidious laboratory growth requirements suggest. Its ability to utilise sialic acid is reliant on a large nan gene cluster located over an 16kb section of its genome (Fig 1). This cluster contains all the genes required for sialic acid catabolism (nanA, nanE), using a putative pathway that is most related to that of the Gastrointestinal (GI) anaerobe Bacteroides fragilis (Brigham), and uptake across both inner (nanT) and outer (nanOU) membranes plus several auxillary genes which most likely play a role in scavenging sialic acid from the environment (nanS, hexA, nanH). Indeed a mutation in the nanH gene abrogates growth of this organism with siallylactose as the sole sialic acid source in biofilm culture (Roy new paper). This operon bears homology both at the sequence level but also at the genome organisation with related human dwelling GI anaerobes such as B. fragilis (BF1711-1720, BF1806-1809) and Parabacteroides distastonis (Figure 1) and represents a new departure from the E. coli paradigm pathway for sialic acid utilisation that dictates the requirement of a neuraminate lyase (NanA), N-acetylmannosamine epimerase (NanE) and an N-acetylmannosamine kinase (NanK) in an operon alongside relevant regulatory, accessory (NanM mutarotase, NanS neuraminate acetyl esterase and transcriptional regulator genes (NanR) (Vimr, Severi) (Fig.1). In E. coli and Haemophilus influenzae NanK is required for phosphorylation of N-acetylmannosamine (ManNac) to ManNac-6P before conversion into N-acetylglucosamine-6-phosphate (NAG-6P). However, in B. fragilis and probably in T. forsythia NanE is capable of converting ManNac to N-acetyl glucosamine, which is then phosphorylated by a hexokinase called RokA (Tf1997 in TF) before being processed by the rest of the pathway (Brigham et al., Roy et al.). Evidence discussed below also suggests that this group of organisms have also adapted a TonB dependent transport module to deal with sialic acid.

Figure 1
Sialic acid catabolism and transport clusters from a range of bacteria

In view of the ability of T. forsythia in particular to use sialic acid one asks the question why and how can it substitute for NAM? And strikingly, why only in a biofilm? However, at present while one can only speculate why this is the case, it is worth noting that in vivo this organism would probably be present as part of the subgingival plaque biofilm where the ability to utilize sialic acid must confer a competitive advantage for nutrition. We have evidence that nan operon gene expression is not only induced in biofilm but also that several of these genes are induced in the presence of sialic acid (Ref Sumi Thesis). In the absence of a putative regulator in this region of the chromosome the route for this regulation is unclear. The ability of this notoriously fastidious bacterium to substitute sialic acid for NAM at all is almost as intriguing as its ability to utilize NAM in the first place (Wyss). Our hypothesis is that T. forsythia might use sialic acid as a means to produce N-acetylmuramic acid (NAM, MurNAc) from N-acetylglucosamine but in a presumably inefficient manner since the amount of sialic acid that supports T. forsythia growth in biofilm (6mM) is far in excess of the 170μM NAM that is equivalent (Roy et al). In E. coli, NagE (GlcNAc-specific phosphotransferase enzyme) (Plumbridge, 2009) and NagZ (N-acetylglucosaminidase) (Dahl et al., 2004) are responsible for converting N-acetylglucosamine (NAG) to N-acetylmuramic acid (NAM) while Bacillus subtilis uses NagE and MurP (MurNAc-specific phosphotransferase system (PTS) ) to yield NAM that it converts into MurNAc-6-phosphate using the NagZ orthologue MurQ (MurAc-6-phosphate-esterase) (Litzinger et al., 2010). However, T. forsythia does not contain any homologues of NagE, MurP or MurQ and also lacks the two step NAM synthesis pathway which includes UDP-N-acetylglucosamine-enolpyruvate transferase and UDP-enolpyruvate reductase (Kolenbrander book: A. Sharma, Genome functions of Tannerella forsythia in bacterial communities. in: P.E. Kolenbrander, (ed.) Oral Microbial Communities: Genome inquiry and interspecies communication, American Society for Microbiology, Washington, D. C., 2011.). Even more intriguing is how exogenous NAM is transported and utilized by T. forsythia in the absence of a putative PTS-like amino sugar uptake system. This suggest that the NAM (and sialic) metabolic pathways in T. forsythia are likely to utilize completely novel pathways and enzymes for sugar transport and utilization of these sugars. The identification and characterization of these pathways will be a focus of our laboratories and others in the near future.

Other periodontal pathogens

While several studies on the periodontal pathogen Porphyromonas gingivalis have highlighted a role for sialic acid in adhesion to human cells – refs from dunhill grant, there is no evidence that it utilises sialic acid as a growth substrate nor contains any catabolic genes in its genome sequence. A similar story also seems to hold true for the fellow red-complex periodontal pathogen Treponema denticola with both possessing at least one putative sialidase encoding gene (genome and Fletcher).

Among other periodontal bacteria the presence of sialic acid catabolic and scavenging genes is varied. For example, the Gram-negative periodontal pathogen Aggregatibacter actinomyceteconcomitans (Aa contains a putative nan operon, while the orange complex bridging organism Fusobacterium nucleatum harbours a full sialic acid utilisation operon comparable to that of H. influenzae with catabolic, inner membrane transport (TRAP type, see below) and regulatory genes clustered together (Figure 1). In addition F. nucleatum has previously been shown to be able to utlilise Sialic acid as a sole carbon source - REF. However, the closely related oral species Fusobacterium nucleatum subsp polymorphum has no catabolic genes but does contain an lst operon that is potentially involved in LPS sialylation (see below). These observations are intriguing given that F. polymorphum is known to form synergistic biofilms with T. forsythia via co-aggregation dependent mechanisms- implying that T. forsythia may adhere to the F. polymorphum surface and scavenge its sialic acid for growth (Ashu synergy paper. In this regard, NanH sialidase is likely to be involved in binding to and cleaving F. polymorphum surface sialic acid residues. In support of this we have preliminary data suggesting that F. polymorphum coaggregates less readily with our T. forsythia nanH mutant than the wild-type and that its LPS banding pattern is altered after sialidase treatment in vitro (unpublished data). Thus it may be that sialic acid also plays a role in nutritional and physical interactions between bacteria known to cohabit within subgingival biofilm. These interactions may also contribute to fitness in vivo as removal of sialic acid from the lipooligosaccharide of H. Influenzae and Neisseria meningitidis by the NanA sialidase of Streptococcus pneumoniae has been suggested to contribute to survival in the respiratory tract during co-infections (Shaknovich et al., 2002).

Diversity of membrane transport systems in periodontal bacteria

Despite the obvious requirement for largely similar biochemical pathways for sialic acid utilisation in pathogenic bacteria, i.e. a mechanism to breakdown sialic acid and assimilate into biomass, there is much more diversity in the mechanisms by which sialic is transported from the extracellular to intracellular environments.

Transit of sialic acid across the inner membrane has been studied in some detail with the identification of dedicated uptake systems that fall into two main categories: 1) Major Facilitator permeases, commonly annotated as NanT (Vimr, Kalivoda, Deszo, & Steenbergen, 2004) and 2) the siaPQM Tripartite ATP-independent periplasmic (TRAP) transporters (Steenbergen et al., 2005) (Figure 2). Recently a third type of sialic acid transporter was discovered in Salmonella typhimurium which is predicted to be present in a range of pathogenic bacteria and is a member of the sodium solute symporter (SSS) family (Severi et al., 2010), while the SatABCD system seems to be limited to Haemophilus ducreyi (ref). Our published work has established that the NanT type permease of T. forsythia is functional upon transplantation into E. coli (ref). Bioinformatic searches of completed genomes reveals that in addition to sharing similar sialic acid catabolic pathways with enteric Bacteroides species, the sialic acid transport systems also seem to be related. Both possess a NanT type permease and notably the novel TonB-dependent NanOU outer membrane transporters also identified in T. forsythia (Figure 1). The other major type of sialic acid inner membrane transport system is of the TRAP family, the mechanisms of which have been revealed in classic biochemical studies by several groups (reviewed by Thomas…). It is in fact this TRAP type transporter that is present in F. nucleatum and Aa (Figure 1,,22).

Figure 2
Summary of sialic acid uptake systems present in periodontal bacteria

In contrast to the inner membrane transport systems, only two sialic acid specific outer membrane transporters have been identified to date. The first was the NanC sialic acid-specific outer membrane porin from E. coli K-12, which is essential for growth on Neu5Ac when the general porins, OmpF, OmpC are not expressed (Condemine et al., 2005). In the genomes of periodontal bacteria sequenced to date, NanC homologues have not been identified thereby leading to the assumption that a general porin may perform this function in these organisms (Figure 2). Our recent work identified the functionality of a second type of outer membrane sialic acid transport system in the T. forsythia sialic acid operon, encoded by the genes NanO and NanU (TF0033 and TF0034), that are able to complement sialic acid growth defects in an E. coli strain devoid of sialic acid transport across its outer membrane (Roy). The protein encoded by nanO is a member of the TonB-dependent receptor family, a class of protein that is often involved in small molecule transport in a mechanism that is energised by the TonB-ExbB-ExbD (TBDR) protein complex (unpublished Roy). Such complexes are typically involved in iron transport but are becoming increasingly recognised as having a role in sugar transport in a range of organisms (3,44,47) and in signal transduction and transcription via ECF-type sigma factors (19). In fact, T. forsythia contains over 60 TBDRs in a genome of 3.4 Mbp and three TonB homologues (1). Therefore, according to the definition of Blanvillain et al., (3) where a ratio of >5 indicates over-representation, the genome of T. forsythia would be considered to be over-represented for TBDRs with a TBDR/Mbp ratio of at least 17.6. This compares with genomes of other members of the Bacteroidetes group where TBDRs are also over-represented (e.g B. fragilis and B. thetaiotaomicron have ratios of 17.7 and 19.1, respectively) (3). The overrepresentation in B. fragilis and Bacteroides thetaiotaomicron is considered an evolutionary adaptation to the gut environment in which they reside, with these TBDRs probably allowing them to acquire and sense a large range of host and dietary carbohydrate molecules (32,44,45,47). These putative TBDRs are also often accompanied in their genome by associated ECF-sigma and anti-sigma factors (19,32,44,45,47), and a preliminary survey of the genome of T. forsythia reveals that 12 of the TBDRs have partner ECF sigma and anti-sigma factors directly adjacent to them on the chromosome. These data indicate that the identified NanO TBDR, that seems to be specific for sialic acid, is part of a much larger repertoire of TBDRs that may well play a similar role for T. forsythia in the oral environment where it is also exposed to a range of dietary sugars as they do for gut Bacteroides.

In contrast to NanO, NanU has homology to other members of the SusD family, that are all predicted to be involved in nutrient utilisation of a range of carbohydrates, often oligomeric (24). It possesses a Type II Signal Recognition particle signal (18) and has predicted structural similarity to the SusD protein from B. thetaiotaomicron (Stafford, G.P. unpublished data). There are a number of homologues of this gene in the T. forsythia genome (1), again indicating that like other Bacteroides species this may be an important mechanism for nutrient uptake. Notably the genomes of several members of the Bacteroidetes also seem to possess homologues of this transport system including B. fragilis (NCTC 9343; BF1719 and 1720) and Parabacteroides diastonis (ATCC 8503: BD_2944 and 2945) both which are adjacent to sialic acid catabolic genes (Figure 1). This indicates that this type of sialic acid uptake system is present in a range of species and may therefore be important not only in the oral cavity but also in the gut. In addition, several other Bacteroides species (e.g. B. thetaiotaomicron) seem to possess NanOU homologues that are associated with fucosidase genes indicating that this family of transporter may also be involved in fucose uptake.

Clearly many questions remain regarding the mechanism of sialic acid transport via the NanOU system such as the putative protein interactions between NanO and NanU, the binding of sialic acid to NanU and the role of TonB in energising the process, which are all currently under investigation in our groups.

Sialidases appear to be multifunctional virulence factors for T. forsythia and other periodontal pathogens

The ability of periodontal pathogens to utilise sialic acid as a growth substrate is particularly pertinent given the range of sialylated glycoproteins present both in oral secretions (e.g. mucins) and on the surface of epithelial cells (e.g. fibronectin, integrins, Toll-like receptors). However, this sialic acid is not freely available and bacteria often employ secreted or membrane-bound sialidase enzymes to capture this sialic acid (Corfield, 1992). In common with many human dwelling organisms T. forsythia and several other periodontal pathogens such as P. gingivalis possess sialidase activity (Moncla, Braham, Hillier 1990) and many of the sequenced stains contain predicted nanH genes (Figure 1).

The T. forsythia NanH protein is most closely related to the sialidase of B. fragilis (65% identity) containing typical sialidase motifs (Roggentin et al., 1993), (Thompson), in addition to a typical putative secretion signal sequence. Recent work has shown that the T. forsythia NanH is able to cleave a2,3 and a2,6 sialyl bonds from both the model sugar siallyllactose (Thompson), the glycoprotein fetuin (Roy- Submitted) and sialic acid lectin binding epitopes from the surface of Ginigival epithelial cells (Honma paper), all suggesting T. forsythia can recover sialic acid from host-glycoproteins and that it is a significant virulence factor. In support of this we now have evidence that fetuin, mucin (less efficiently), and saliva support growth of T. forsythia in a sialic acid dependent manner- i.e. growth is inhibited by sialidase inhibitors and by loss of the nanH gene by mutation (Roy et al., in submission). The idea that sialidases may be important in vivo for periodontal bacteria is not unexpected given that a nanH mutant of the related organism B. fragilis displayed reduced colonisation ability in rats (Godoy et al., 1993) and that the periodontal pocket in which periodontal pathogens reside contains an abundance of sialylated glycoproteins (Pollanen et al., 2003). Notably, all Bacteroidetes members that contain a nan-operon linked nanH gene also contain a putative 9-O-acetylesterase enzyme for the removal of the acetyl group at position 9 of sialic acid which is known to inhibit sialidase enzymes ref also (Figure 1). Presumably this implies that 9-O-acetylated sialic acid, which makes up a large proportion of the sialic acid in the human body e.g. 80% of the sialic acid content of mucins is o-acetylated (Varki and Diaz, 1983), is an important source of sialic acid for these organisms. This is not completely surprising since the enteric human bacterium E. coli has an 9-O-acetylesterase (NanS) which has specificity towards 9-O-acetyl sialic acid as a growth substrate (Steenbergen et al., 2009). In contrast P. gingivalis which possesses a true sialidase (PG0352) and two O-sialoglycoproteases (PG0778 & PG1724), but no metabolic genes, does not, but may be able to cleave these linkages via the sialoglycoproteases (Fletcher).

Bacterial sialidase enzymes also play an important role in adherence to both human cells and solid surfaces (Corfield, 1992). For example, the lectin-like Streptococcus pneumoniae sialidase, NanA, is important for adherence to endothelial cells (Uchiyama et al., 2009) while our work on T. forsythia highlighted a role in adhesion to oral epithelial cells (Honma) and others also showed that interaction of T. forsythia with blood cells can be inhibited by sialyllactose (Murakami et al., 2002). The picture for Porphyromonas gingivalis is less clear with various sialidase and sialoglycoprotease mutants having different adhesion and invasion phenotypes (Fletcher) while pretreatment of human cells with sialidase or incubation with sialic acid reduces invasion (Hallen, Agnani). The importance of sialic acid in host interactions of periodontal pathogens though is clear and holds true for the final Red complex organism T. denticola whose interaction with erythrocytes is inhibited by low concentrations of sialic acid (Mixk paper).

In addition to interactions with host cells (and secretions in the oral cavity periodontal pathogens also display various interactions with other oral bacteria and in the formation of biofilm evidence body of data now suggesting a role for sialic acid and sialidases here too. For example pretreatment of P. gingivalis with sialidase enzyme reduces interactions with Streptococcus sanguis (Stinson) and we have preliminary evidence that a nanH mutant of T. forsythia aggregates less readily with the putatively sialic acid coated important bridging organism F. polymorphum (Bolstad et al., 1996), suggesting a nutritional and physical basis for their synergistic relationship (Sharma). Our recent work has also highlighted that T. forsythia sialidase is key to adhesion and biofilm formation on glycoprotein coated surfaces (Roy et al., unpub.), an observation that reflects a possible role in colonization of epithelial surfaces as was observed for the important respiratory pathogens Pseudomonas aeruginosa (Soong et al., 2006) and S. pneumoniae (Parker, Soong, Planet, Brower, Ratner & Prince, 2009) King et al., 2006 (Krivan et al., 1988). The role of sialidase in these cases may be both as an adhesin itself (many have lectin like properties) but also in the exposure of underlying adhesive epitopes such as galactose, as is the case for Bacteroides intermedius, Actinomyces spp. and Vibrio cholera toxins (Okuda et al., 1989; Gibbons et al., 1990; Moustafa et al., JBC 2004). With respect to colonization by F. nucleatum in the oral cavity, sialidases could be involved in exposing galactose residues on host surfaces for subsequent binding by lectin-like adhesin expressed by the bacterium (Infect. Immun. 56 (1988) 1314-1319).

In addition to providing nutritional and adhesive function to organisms it is increasingly recognized that sialidases play roles in modulation of the immune responses and immune evasion. For example, S. pneumoniae sialidase initiates the extensive deglycosylation of secretory component and IgA1 (Molecular Microbiology (2006) (3), 961–974) as well as serum glycoproteins that results in reduced complement deposition and subsequent inhibition of killing by neutrophils (IAI 78:2108). Recent studies have also reported that full activation of Toll-like receptors in macrophage and endothelial cells that are key to the innate immune response following exposure to infectious agent molecules such as LPS, Teichoic acid and flagella is dependent on the function of host sialidases but that this can also be modified by bacterially derived sialidases with specificity for alpha 2,3 linkages (Amith, Glycoconj. J. 2009, 26:1197; Amith, Cell Signal 2010, 22:314; Stomatos et al., J. Leukoc. Biol. 2010 88: 1227). These data also suggest that the alpha 2,3 and 2,6 specific sialidases of periodontal bacteria may also contribute to the pathologic effects observed in host epithelial layers.

The potential for surface coating and host mimicry

Whether sialic acid is de novo synthesized or obtained exogenously by the action of sialidases on host and/or cohabiting bacterial glyconjugates, many pathogens are able to decorate their surface molecules (LPS and capsular polysaccharide) with sialic acids and its derivatives in order to mimic host cell surfaces, e.g. H. influenzae, N. meningitidis, E. coli K1 and C. jejuni (refs). This ‘molecular mimicry’ helps in the avoidance of host immune attack (Vimr and Lichtensteiger, 2002, Severi). Moreover, surface sialic acid expression is also believed to hinder the reach of complement dependent membrane attack complex (MAC on the bacterial membrane. With regard to bactera of the oral cavity, the F. polymorphum genome possesses (www.oralgen.org) putative genes (FNP_1104-FNP_1109 ) which encodes neuC, neuA, neuB, N-acetylneuraminate synthase, a polysaccharide biosynthesis export protein and lipooligosaccharide sialyltransferase (lst respectively (http://www.oralgen.lanl.gov/) that might be involved in sialylation of LPS. It remains to be seen whether LPS sialylation does occur in F. polymorphum and if it plays any role in immune mimicry.

However, to date, no other periodontal bacteria with gene homologues indicating LPS sialylation have been reported/documented.

In addition to LPS and capsule sialylation it is becoming clear that many bacteria are capable of glycosylation of surface proteins such as flagella (refs) and outer membrane proteins (bfrag refs). One such example is the S-layer of T. forsythia which is known to be heavily glycosylated. However, the nature of this glycosylation is unclear and the only hints as to its composition come from a mutation in a putative N-acetyl-D-mannosaminuronic acid dehydrogenase gene (wecC that results in a defect in protein glycosylation (Honma ref) and in the presence of several potential fucosylation sites of the type identified in B. fragilis by xxxxx (ref). While there is no evidence of sialylation of the S-layer thus far it is tempting to speculate that this might be the case, especially given that the related organism P. gingivalis (which also lacks sialylation pathways) contains sialic acid attached to the major surface gingipain, Rgp (Rangarajan). One possible mechanism for this could be trans-sialylation via sialidase enzymes as has been observed in eukaryotes in recent years.

Interestingly, the molecular mimicry reaches near perfection in another human commensal/pathogen, the Group B Streptococci (GBS). GBS express capsular polysaccharide that displays the glycan structure Siaα-2-3Galβ1-4GlcNac, found in human glycoproteins recognized by Sia-recognizing immunoglobulin superfamily lectin (Siglecs) receptors on immune cells, including neutrophils. Siglec receptors, due to the presence of cytoplasmic tyrosine based inhibitory motif (ITIM are believed to dampen the inflammatory responses following host sialoglycan binding. Thus, molecular mimicy by GBS has been postulated to impair neutrophil defense functions by coopting Siglecs. The interaction with Siglecs can also can lead to other consequences, such as in the case of C. jejnui where reconition of sialic acid containing antigens contributes to autoimmune disorders such as Guillan-Barre syndrome (Carlin et al., 2007;Ilg et al., 2010).

Whether any or all of these phenomena are present in periodontal pathogens is not clear at present but there is clearly much scope for this to be the case, a fact that would add to the complexity of interactions between these bacteria and the host immune system.

A Model for the role of sialic acid in periodontitis

Overall there is a growing body of evidence and opinion that sialic acid plays a key role in the life and consequences of periodontal pathogen colonisation (Figure 3). There seems little doubt that at least for some periodontal organisms sialic acid can act as a growth and adhesion factor for colonisation both of host surfaces but also probably in interactions with other oral dwelling bacteria within biofilm. The production of sialidase enzymes by a range of periodontal pathogens and the obvious potential therefore for modulation of the immune response means there is much to investigate and much scope for the design of new treatment regimen as well as an increased understanding of the microbial community in the oral cavity.

Figure 3
Model of sialic acid dependent interactions of periodontal pathogens

Acknowledgements

Work in the Stafford Group is funded by a Dunhill Medical Trust Grant to GS and a Wellcome Trust VIP award to SR. The Sharma Group is funded by by U.S. Public Health Grant DE014749. We would also like to thank Drs Jennifer Parker and Prachi Stafford for critical reading of this document.

REFERENCES

  • Agnani G, Tricot-Doleux S, Du L, Bonnaure-Mallet M. Adherence of Porphyromonas gingivalis to gingival epithelial cells: modulation of bacterial protein expression. Oral Microbiol Immunol. 2000;15:48–52. [PubMed]
  • Amith SR, Jayanth P, Franchuk S, et al. Dependence of pathogen molecule-induced toll-like receptor activation and cell function on Neu1 sialidase. Glycoconj J. 2009;26:1197–1212. [PubMed]
  • Amith SR, Jayanth P, Franchuk S, et al. Neu1 desialylation of sialyl alpha-2,3-linked beta-galactosyl residues of TOLL-like receptor 4 is essential for receptor activation and cellular signaling. Cell Signal. 2010;22:314–324. [PubMed]
  • Aruni W, Vanterpool E, Osbourne D, et al. Siali-dase and sialoglycoproteases can modulate virulence in Porphyromonas gingivalis. Infect Immun. 2011;79:2779–27791. [PMC free article] [PubMed]
  • Blanvillain S, Meyer D, Boulanger A, et al. Plant carbohydrate scavenging through tonb-dependent receptors: a feature shared by phytopathogenic and aquatic bacteria. PLoS ONE. 2007;2:e224. [PMC free article] [PubMed]
  • Bolstad AI, Jensen HB, Bakken V. Taxonomy, biology, and periodontal aspects of Fusobacterium nucleatum. Clin Microbiol Rev. 1996;9:55–71. [PMC free article] [PubMed]
  • Brigham CJ, Malamy MH. Characterization of the RokA and HexA broad-substrate-specificity hexo-kinases from Bacteroides fragilis and their role in hexose and N-acetylglucosamine utilization. J Bacteriol. 2005;187:890–901. [PMC free article] [PubMed]
  • Brigham C, Caughlan R, Gallegos R, Dallas MB, Godoy VG, Malamy MH. Sialic acid (N-acetyl neuraminic acid) utilization by Bacteroides fragilis requires a novel N-acetyl mannosamine epimerase. J Bacteriol. 2009;191:3629–3638. [PMC free article] [PubMed]
  • Carlin AF, Lewis AL, Varki A, Nizet V. Group B streptococcal capsular sialic acids interact with siglecs (immunoglobulin-like lectins) on human leukocytes. J Bacteriol. 2007;189:1231–1237. [PMC free article] [PubMed]
  • Chou WK, Dick S, Wakarchuk WW, Tanner ME. Identification and characterization of NeuB3 from Campylobacter jejuni as a pseudaminic acid synthase. J Biol Chem. 2005;280:35922–35928. [PubMed]
  • Condemine G, Berrier C, Plumbridge J, Ghazi A. Function and expression of an N-acetylneurami-nic acid-inducible outer membrane channel in Escheri-chia coli. J Bacteriol. 2005;187:1959–1965. [PMC free article] [PubMed]
  • Corfield T. Bacterial sialidases – roles in pathogenicity and nutrition. Glycobiology. 1992;2:509–521. [PubMed]
  • Curtis MA, Hanley SA, Aduse-Opoku J. The rag locus of Porphyromonas gingivalis: a novel pathogenicity island. J Periodontal Res. 1999;34:400–405. [PubMed]
  • Dahl U, Jaeger T, Nguyen BT, Sattler JM, Mayer C. Identification of a phosphotransferase system of Escherichia coli required for growth on N-acetylmuramic acid. J Bacteriol. 2004;186:2385–2392. [PMC free article] [PubMed]
  • Dalia AB, Standish AJ, Weiser JN. Three surface exoglycosidases from Streptococcus pneumo-niae, NanA, BgaA, and StrH, promote resistance to op-sonophagocytic killing by human neutrophils. Infect Immun. 2010;78:2108–2116. [PMC free article] [PubMed]
  • Fischer M, Zhang QY, Hubbard RE, Thomas GH. Caught in a TRAP: substrate-binding proteins in secondary transport. Trends Microbiol. 2010;18:471–478. [PubMed]
  • Fletcher CM, Coyne MJ, Comstock LE. Theoretical and experimental characterization of the scope of protein O-glycosylation in Bacteroides fragilis. J Biol Chem. 2011;286:3219–3226. [PMC free article] [PubMed]
  • Gibbons RJ, Hay DI, Childs WC, III, Davis G. Role of cryptic receptors (cryptitopes) in bacterial adhesion to oral surfaces. Arch Oral Biol. 1990;35(Suppl):107S–114S. [PubMed]
  • Godoy VG, Dallas MM, Russo TA, Malamy MH. A role for Bacteroides fragilis neuramini-dase in bacterial growth in two model systems. Infect Immun. 1993;61:4415–4426. [PMC free article] [PubMed]
  • Hallen U, Angstrom J, Bjorkner AE. Glyco-lipid binding epitopes involved in adherence of the peri-odontitis-associated bacterium Porphyromonas gingivalis. Glycoconj J. 2008;25:561–572. [PubMed]
  • Honma K, Inagaki S, Okuda K, Kuramitsu HK, Sharma A. Role of a Tannerella forsythia exo-polysaccharide synthesis operon in biofilm development. Microb Pathog. 2007;42:156–166. [PubMed]
  • Honma K, Mishima E, Sharma A. Role of Tannerella forsythia NanH sialidase in epithelial cell attachment. Infect Immun. 2011;79:393–401. [PMC free article] [PubMed]
  • Hood DW, Cox AD, Gilbert M, et al. Identification of a lipopolysaccharide alpha-2,3-sialyltransferase from Haemophilus influenzae. Mol Microbiol. 2001;39:341–350. [PubMed]
  • Ilg K, Yavuz E, Maffioli C, Priem B, Aebi M. Glycomimicry: display of the GM3 sugar epitope on Escherichia coli and Salmonella enterica sv Typhimurium. Glycobiology. 2010;10:1289–1297. [PubMed]
  • Juncker AS, Willenbrock H, Von Heijne G, Brunak S, Nielsen H, Krogh A. Prediction of lipo-protein signal peptides in Gram-negative bacteria. Protein Sci. 2003;12:1652–1662. [PubMed]
  • Kapatral V, Anderson I, Ivanova N, et al. Genome sequence and analysis of the oral bacterium Fuso-bacterium nucleatum strain ATCC 25586. J Bacteriol. 2002;184:2005–2018. [PMC free article] [PubMed]
  • King SJ, Hippe KR, Weiser JN. Degly-cosylation of human glycoconjugates by the sequential activities of exoglycosidases expressed by Streptococcus pneumoniae. Mol Microbiol. 2006;59:961–974. [PubMed]
  • Koebnik R. TonB-dependent trans-envelope signalling: the exception or the rule? Trends Microbiol. 2005;13:343–347. [PubMed]
  • Krivan HC, Roberts DD, Ginsburg V. Many pulmonary pathogenic bacteria bind specifically to the carbohydrate sequence GalNAc beta 1-4Gal found in some glycolipids. Proc Natl Acad Sci USA. 1988;85:6157–6161. [PubMed]
  • Litzinger S, Duckworth A, Nitzsche K, Risinger C, Wittmann V, Mayer C. Muropeptide rescue in Bacillus subtilis involves sequential hydrolysis by beta-N-acetylglucosaminidase and N-acetylmuramyl-l-alanine amidase. J Bacteriol. 2010;192:3132–3143. [PMC free article] [PubMed]
  • Mikx FH, Keulers RA. Hemagglutination activity of Treponema denticola grown in serum-free medium in continuous culture. Infect Immun. 1992;60:1761–1766. [PMC free article] [PubMed]
  • Moncla BJ, Braham P, Hillier SL. Sialidase (neuraminidase) activity among gram-negative anaerobic and capnophilic bacteria. J Clin Microbiol. 1990;28:422–425. [PMC free article] [PubMed]
  • Murakami Y, Higuchi N, Nakamura H, Yoshimura F, Oppenheim FG. Bacteroides forsythus hemagglutinin is inhibited by N-acetylneuraminyllactose. Oral Microbiol Immunol. 2002;17:125–128. [PubMed]
  • Murray PA, Kern DG, Winkler JR. Identification of a galactose-binding lectin on Fusobacterium nucleatum FN-2. Infect Immun. 1988;56:1314–1319. [PMC free article] [PubMed]
  • Nagano K, Murakami Y, Nishikawa K, Sakakibara J, Shimozato K, Yoshimura F. Characterization of RagA and RagB in Porphyromonas gingivalis: study using gene-deletions mutants. J Med Microbiol. 2007;56:1536–1548. [PubMed]
  • Okuda K, Ono M, Kato T. Neuraminidase-enhanced attachment of Bacteroides intermedius to human erythrocytes and buccal epithelial cells. Infect Immun. 1989;57:1635–1637. [PMC free article] [PubMed]
  • Oral Pathogen Sequence Database Oral pathogen sequence databases [Internet] 2008 Available from: http://www.oralgen.lanl.gov/
  • Parker D, Soong G, Planet P, Brower J, Ratner AJ, Prince A. The NanA neuraminidase of Streptococcus pneumoniae is involved in biofilm formation. Infect Immun. 2009;77:3722–3730. [PMC free article] [PubMed]
  • Plumbridge J. An alternative route for recycling of N-acetylglucosamine from peptidoglycan involves the N-acetylglucosamine phosphotransferase system in Escherichia coli. J Bacteriol. 2009;191:5641–5647. [PMC free article] [PubMed]
  • Pollanen MT, Salonen JI, Uitto VJ. Structure and function of the tooth-epithelial interface in health and disease. Periodontol 2000. 2003;31:12–31. [PubMed]
  • Post DM, Mungur R, Gibson BW, Munson RS., Jr Identification of a novel sialic acid transporter in Haemophilus ducreyi. Infect Immun. 2005;73:6727–6735. [PMC free article] [PubMed]
  • Rangarajan M, Hashim A, Aduse-Opoku J, Paramonov N, Hounsell EF, Curtis MA. Expression of Arg-Gingipain RgpB is required for correct glycosylation and stability of monomeric Arg-gingipain RgpA from Porphyromonas gingivalis W50. Infect Immun. 2005;73:4864–4878. [PMC free article] [PubMed]
  • Reeves AR, Wang GR, Salyers AA. Characterization of four outer membrane proteins that play a role in utilization of starch by Bacteroides thetaiotaomicron. J Bacteriol. 1997;179:643–649. [PMC free article] [PubMed]
  • Roggentin P, Schauer R, Hoyer LL, Vimr ER. The sialidase superfamily and its spread by horizontal gene transfer. Mol Microbiol. 1993;9:915–921. [PubMed]
  • Roy S. Biofilm Formation and Sialic acid Utilisation by Tannerella forsythia [PhD thesis] The University of Sheffield; Sheffield, UK: 2010.
  • Roy S, Douglas CW, Stafford GP. A novel sialic acid utilization and uptake system in the periodontal pathogen Tannerella forsythia. J Bacteriol. 2010;192:2285–2293. [PMC free article] [PubMed]
  • Roy S, Honma K, Douglas I, Sharma A, Stafford GP. Role of sialidase in glycoprotein utilisation by Tannerella forsythia. Microbiology. 2011;157:3195–3202. [PMC free article] [PubMed]
  • Severi E, Hood DW, Thomas GH. Sialic acid utilization by bacterial pathogens. Microbiology. 2007;153:2817–2822. [PubMed]
  • Severi E, Hosie AHF, Hawkhead JA, Thomas GH. Characterization of a novel sialic acid transporter of the sodium solute symporter (SSS) family and in vivo comparison with known bacterial sialic acid transporters. FEMS Microbiol Lett. 2010;304:47–54. [PubMed]
  • Shakhnovich EA, King SJ, Weiser JN. Neuraminidase expressed by Streptococcus pneumo-niae desialylates the lipopolysaccharide of Neisseria meningitidis and Haemophilus influenzae: a paradigm for interbacterial competition among pathogens of the human respiratory tract. Infect Immun. 2002;70:7161–7164. [PMC free article] [PubMed]
  • Sharma A. Genome functions of Tannerella for-sythia in bacterial communities. In: Kolenbrander PE, editor. Oral Microbial Communities: Genome Inquiry and Interspecies Communication. American Society for Microbiology; Washington, DC: 2011. pp. 135–147.
  • Sharma A, Inagaki S, Sigurdson W, Kuramitsu HK. Synergy between Tannerella forsythia and Fusobacterium nucleatum in biofilm formation. Oral Microbiol Immunol. 2005;20:39–42. [PubMed]
  • Shell DM, Chiles L, Judd RC, Seal S, Rest RF. The Neisseria lipooligosaccharide-specific alpha-2,3-sialyltransferase is a surface-exposed outer membrane protein. Infect Immun. 2002;70:3744–3751. [PMC free article] [PubMed]
  • Socransky SS, Haffajee AD, Cugini MA, Smith C, Kent RL., Jr Microbial complexes in subgingival plaque. J Clin Periodontol. 1998;25:134–144. [PubMed]
  • Sonnenburg JL, Xu J, Leip DD, et al. Glycan foraging in vivo by an intestine-adapted bacterial symbiont. Science. 2005;307:1955–1959. [PubMed]
  • Soong G, Muir A, Gomez MI, et al. Bacterial neuraminidase facilitates mucosal infection by participating in biofilm production. J Clin Invest. 2006;116:2297–2305. [PubMed]
  • Stamatos NM, Carubelli I, van de Vlekkert D, et al. LPS-induced cytokine production in human dendritic cells is regulated by sialidase activity. J Leukoc Biol. 2010;88:1227–1239. [PubMed]
  • Steenbergen SM, Lichtensteiger CA, Caughlan R, Garfinkle J, Fuller TE, Vimr ER. Sialic acid metabolism and systemic pasteurellosis. Infect Immun. 2005;73:1284–1294. [PMC free article] [PubMed]
  • Steenbergen SM, Jirik JL, Vimr ER. YjhS (NanS) is required for Escherichia coli to grow on 9-O-acetylated N-acetylneuraminic acid. J Bacteriol. 2009;191:7134–7139. [PMC free article] [PubMed]
  • Stinson MW, Safulko K, Levine MJ. Adherence of Porphyromonas (Bacteroides) gingivalis to Streptococcus sanguis in vitro. Infect Immun. 1991;59:102–108. [PMC free article] [PubMed]
  • Thompson H, Homer KA, Rao S, Booth V, Hosie AH. An orthologue of Bacteroides fragilis NanH is the principal sialidase in Tannerella forsythia. J Bacteriol. 2009;191:3623–3628. [PMC free article] [PubMed]
  • Uchiyama S, Carlin AF, Khosravi A, et al. The surface-anchored NanA protein promotes pneumococcal brain endothelial cell invasion. J Exp Med. 2009;206:1845–1852. [PMC free article] [PubMed]
  • Varki A, Diaz S. A neuraminidase from Streptococcus sanguis that can release O-acetylated sialic acids. J Biol Chem. 1983;258:12465–12471. [PubMed]
  • Varki A, Schauer R. Sialic acids. In: Varki A, Cummings RD, Esko JD, editors. Essentials of Glycobiology. Cold Spring Harbor Laboratory Press; New York: 2009. pp. 199–218.
  • Vimr E, Lichtensteiger C. To sialylate, or not to sialylate: that is the question. Trends Microbiol. 2002;10:254–257. [PubMed]
  • Vimr ER, Kalivoda KA, Deszo EL, Steenbergen SM. Diversity of microbial sialic acid metabolism. Microbiol Mol Biol Rev. 2004;68:132–153. [PMC free article] [PubMed]
  • Wyss C. Dependence of proliferation of Bacteroides-forsythus on exogenous N-acetylmuramic acid. Infect Immun. 1989;57:1757–1759. [PMC free article] [PubMed]
  • Xu J, Gordon JI. Inaugural article: honor thy symbionts. Proc Natl Acad Sci USA. 2003;100:10452–10459. [PubMed]
  • Xu J, Bjursell MK, Himrod J, et al. A genomic view of the human-Bacteroides thetaiotaomicron symbiosis. Science. 2003;299:2074–2076. [PubMed]
  • Xu J, Chiang HC, Bjursell MK, Gordon JI. Message from a human gut symbiont: sensitivity is a prerequisite for sharing. Trends Microbiol. 2004;12:21–28. [PubMed]
  • Xu J, Mahowald MA, Ley RE, et al. Evolution of symbiotic bacteria in the distal human intestine. PLoS Biol. 2007;5:e156. [PubMed]