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Media- and method-dependent variations in minimal inhibitory concentrations of antiplaque agents on oral bacteria. - PubMed - NCBI
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Lett Appl Microbiol. 2006 Sep;43(3):256-61.

Media- and method-dependent variations in minimal inhibitory concentrations of antiplaque agents on oral bacteria.

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1
School of Dental Medicine, University at Buffalo, Buffalo, NY 14214-3008, USA.

Abstract

AIMS:

To determine minimal inhibitory concentrations (MICs) and the percentage of nonsusceptible bacteria-- those still cultivable above a threshold concentration--in human supragingival dental plaque and saliva for antiplaque/antimicrobial agents including triclosan (TCS) and trichlorocarbanilide (TCC), and a new potential antimicrobial, 2-t-butyl-5-(4-t-butylphenyl)-phenol (DTBBP).

METHODS AND RESULTS:

Broth and agar dilution-based MIC tests were performed using 28 oral and nonoral bacterial strains representing 17 species. MICs for TCS were lowest and more than 100-fold lower than DTBBP (P < 0.0005) by both methods. MICs for TCS were lower in broth-based tests compared with TCC. The additions of defibrinated blood to agar and horse serum to broth increased MICs--in the case of TCS, 10- to 15-fold. Significantly higher proportions of nonsusceptible plaque and salivary bacteria were recovered from agar media containing DTBBP or TCC compared with TCS (P < 0.05).

CONCLUSIONS:

TCS is a more effective antimicrobial agent than either TCC or DTBBP as determined by in vitro testing.

SIGNIFICANCE AND IMPACT OF THE STUDY:

The utility of in vitro testing for antiplaque agents as a predictor of in vivo efficacy is affected by the methods used.

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