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Construction of recombination-deficient strains of Streptococcus gordonii by disruption of the recA gene. - PubMed - NCBI
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J Bacteriol. 1993 Oct;175(19):6354-7.

Construction of recombination-deficient strains of Streptococcus gordonii by disruption of the recA gene.

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1
Department of Cariology, School of Dentistry, University of Michigan, Ann Arbor 48109.

Abstract

Degenerate oligonucleotide primers were used in a polymerase chain reaction (PCR) to amplify a region of the recA sequence of Streptococcus gordonii Challis. The resulting PCR fragment was cloned into the suicide vector pAM6199 and introduced into strain Challis, giving rise to recombination-deficient strains in which the recA gene was specifically inactivated.

PMID:
8407809
PMCID:
PMC206735
[Indexed for MEDLINE]
Free PMC Article
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