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Development of a modified gentamicin resistance cassette for genetic manipulation of the oral spirochete Treponema denticola. - PubMed - NCBI
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Appl Environ Microbiol. 2012 Mar;78(6):2059-62. doi: 10.1128/AEM.07461-11. Epub 2012 Jan 13.

Development of a modified gentamicin resistance cassette for genetic manipulation of the oral spirochete Treponema denticola.

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1
Department of Oral Biology, State University of New York at Buffalo, Buffalo, New York, USA.

Abstract

Herein, we report that a modified gentamicin cassette and a PCR-based method can be used for targeted mutagenesis of the oral spirochete Treponema denticola. This approach minimizes polar effects and spontaneous antibiotic resistance. Therefore, it can serve as a reliable tool for genetic manipulation of T. denticola.

PMID:
22247130
PMCID:
PMC3298132
DOI:
10.1128/AEM.07461-11
[Indexed for MEDLINE]
Free PMC Article
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