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1.
Fig. 4.

Fig. 4. From: Tannerella forsythia invasion in oral epithelial cells requires phosphoinositide 3-kinase activation and clathrin-mediated endocytosis.

Invasion assay with (a) EHT1864- and (b) C3 transferase-treated KB and OBA-9 cells. Values are expressed as a percentage of bacterial invasion of non-treated KB cells (designated 100 %). Error bars, sd. Asterisks indicate statistically significant differences relative to untreated cells (*, P<0.05; ***, P<0.001).

Elina Mishima, et al. Microbiology. 2011 Aug;157(Pt 8):2382-2391.
2.
Fig. 1.

Fig. 1. From: Tannerella forsythia invasion in oral epithelial cells requires phosphoinositide 3-kinase activation and clathrin-mediated endocytosis.

Invasion assay with (a) genistein-, (b) wortmannin- and (c) LY249002-treated KB and OBA-9 cells. Values are expressed as a percentage of the bacterial invasion of non-treated cells (designated 100 %). Results are means and sds of three independent experiments each with triplicate measurements. ** and *** indicate P<0.01 and P<0.001, respectively, relative to untreated cells.

Elina Mishima, et al. Microbiology. 2011 Aug;157(Pt 8):2382-2391.
3.
Fig. 3.

Fig. 3. From: Tannerella forsythia invasion in oral epithelial cells requires phosphoinositide 3-kinase activation and clathrin-mediated endocytosis.

(a) Relative expression of RhoA, Rac1 and Cdc42, assessed by qRT-PCR. The values were normalized with respect to GAPDH expression. The expression of each gene in non-treated KB cells was designated 100 %. (b) Invasion efficiency of Rac1, Cdc42 and RhoA knockdown KB cells. The number of bacteria that invaded non-treated KB cells was designated 100 %. Non-specific indicates KB cells treated with negative control RNAi. Results are means and sds of three independent experiments each with triplicate measurements. The asterisk indicates a statistically significant difference relative to untreated cells.

Elina Mishima, et al. Microbiology. 2011 Aug;157(Pt 8):2382-2391.
4.
Fig. 6.

Fig. 6. From: Tannerella forsythia invasion in oral epithelial cells requires phosphoinositide 3-kinase activation and clathrin-mediated endocytosis.

(a) Relative expression of CLTC assessed by qRT-PCR and Western immunoblotting. For qRT-PCR, values were normalized with respect to β-actin expression. The CLTC expression of non-treated KB cells was designated 100 % (***, P<0.001). (b) T. forsythia infection efficiency in CLTC knockdown KB cells. The number of bacteria that invaded non-treated KB cells was designated 100 %. Results are means and sems of three independent experiments each with triplicate measurements. Asterisks indicate statistically significant differences relative to untreated cells (**, P<0.01). (c, d) T. forsythia invasion assay with MDC- and chloropromazine-treated KB and OBA-9 cells. The number of bacteria that invaded non-treated KB or OBA-9 cells was designated 100 %. Results are means and sds of three independent experiments each with triplicate measurements. Asterisks indicate statistically significant differences relative to untreated cells (**, P<0.01; ***, P<0.001).

Elina Mishima, et al. Microbiology. 2011 Aug;157(Pt 8):2382-2391.
5.
Fig. 5.

Fig. 5. From: Tannerella forsythia invasion in oral epithelial cells requires phosphoinositide 3-kinase activation and clathrin-mediated endocytosis.

T. forsythia invasion assay with (a) MβCD- and (b) nystatin-treated KB and OBA-9 cells. The number of bacteria that invaded non-treated KB cells was designated 100 %. Results are means and sems of three independent experiments each with triplicate measurements. Asterisks indicate statistically significant differences relative to untreated cells (*, P<0.05; **, P<0.01; ***, P<0.001). (c) Relative expression of Cav-1 was assessed by qRT-PCR and Western immunoblotting. For qRT-PCR, the values were normalized with respect to β-actin expression. The Cav-1 expression of non-treated KB cells was designated 100 %. (d) T. forsythia and P. gingivalis invasion efficiency in Cav-1 knockdown KB cells. The number of bacteria that invaded non-treated KB cells was designated 100 %. Results are means and sems of three independent experiments each with triplicate measurements. *, P<0.05 relative to untreated cells.

Elina Mishima, et al. Microbiology. 2011 Aug;157(Pt 8):2382-2391.
6.
Fig. 2.

Fig. 2. From: Tannerella forsythia invasion in oral epithelial cells requires phosphoinositide 3-kinase activation and clathrin-mediated endocytosis.

Akt kinase activity in KB (a, b) and OBA-9 (c, d) cells following EGF, T. forsythia or BspA treatment. (a, c) Cell extracts were analysed by Western blotting. Anti-Akt and anti-phospho-Akt antibodies were used as primary antibodies. (b, d) Densitometric analyses of immunoblots in (a) and (c) showing expression levels of phospho-Akt after various treatments relative to those of non-treated cells. Total Akt levels were used for normalization. Data were analysed with NIH ImageJ software. The tables below the bar graphs show fold changes observed for representative blots and two other independent replicates.

Elina Mishima, et al. Microbiology. 2011 Aug;157(Pt 8):2382-2391.

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