Doxycycline-mediated quantitative and tissue-specific control of gene expression in transgenic mice

A Kistner, M Gossen, F Zimmermann… - Proceedings of the …, 1996 - National Acad Sciences
Abstract The tet regulatory system in which doxycycline (dox) acts as an inducer of
specifically engineered RNA polymerase II promoters was transferred into transgenic mice.
Tight control and a broad range of regulation spanning up to five orders of magnitude were
monitored dependent on the dox concentration in the water supply of the animals.
Administration of dox rapidly induces the synthesis of the indicator enzyme luciferase …