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Assay Portal | Office of Cancer Clinical Proteomics Research

Overview Data source: UniProt

Official Gene Symbol Other Aliases
TP53 P53
Sequence Length (AA) Molecular Weight (Da)
393 43653
Protein Name
Cellular tumor antigen p53
Sources
UniProt
PhosphoSitePlus ®
GeneCards
Human Protein Atlas

Protein Sequence hover to view complete sequence

10 20 30 40 50
MEEPQSDPSV EPPLSQETFS DLWKLLPENN VLSPLPSQAM DDLMLSPDDI
60 70 80 90 100
EQWFTEDPGP DEAPRMPEAA PPVAPAPAAP TPAAPAPAPS WPLSSSVPSQ
110 120 130 140 150
KTYQGSYGFR LGFLHSGTAK SVTCTYSPAL NKMFCQLAKT CPVQLWVDST
160 170 180 190 200
PPPGTRVRAM AIYKQSQHMT EVVRRCPHHE RCSDSDGLAP PQHLIRVEGN
210 220 230 240 250
LRVEYLDDRN TFRHSVVVPY EPPEVGSDCT TIHYNYMCNS SCMGGMNRRP
260 270 280 290 300
ILTIITLEDS SGNLLGRNSF EVRVCACPGR DRRTEEENLR KKGEPHHELP
310 320 330 340 350
PGSTKRALPN NTSSSPQPKK KPLDGEYFTL QIRGRERFEM FRELNEALEL
360 370 380 390 393
KDAQAGKEPG GSRAHSSHLK SKKGQSTSRH KKLMFKTEGP DSD

Data source: UniProt


Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs

Uniprot Database Entry PhosphoSitePlus ®

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Assay Details for CPTAC-951 Collapse assay details

Data source: Panorama

Official Gene Symbol
TP53
Peptide Modified Sequence
ALPNNTSSS[+80.0]PQPK
Modification Type
Phospho (ST)
Protein - Site of Modification
315
Peptide - Site of Modification
9
Peptide Start
307
Peptide End
319
CPTAC ID
CPTAC-951
Peptide Molecular Mass
1,419.6395
Species
Homo sapiens (Human)
Assay Type
Enrichment MRM
Enrichment Method
IMAC
Matrix
cell lysate
Submitting Laboratory
Fred Hutchinson Cancer Research Center
Submitting Lab PI
Amanda Paulovich

Publication

View Details (opens in a new window)

Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940


Assay Parameters Collapse assay parameters

Data source: Panorama

Instrument
QTrap 5500 (Sciex)
Internal Standard
Peptide
Peptide Standard Purity
>95%
Peptide Standard Label Type
13C and 15N at C-terminus K
LC
nanoLC-2D (Eksigent)
Column Packing
ChromXP C18-CL, 3 µm, 120 Å
Column Dimensions
75 µm x 15cm
Flow Rate
300 nL/min

Chromatograms

Data source: Panorama


Response Curves

Data source: Panorama

Retrieving Data

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Repeatability

Data source: Panorama

  Average intra-assay CV
(within day CV)
Average inter-assay CV
(between day CV)
Total CV
equation
n=
Fragment ion / Transition Low Med High Low Med High Low Med High Low Med High
b8 (1+) 63.8 71.1 45.7 60.3 78.3 101.2 87.8 105.8 111 15 24 15
y10 (2+) 15.7 12.4 10 33.5 14.3 14 37 18.9 17.2 15 24 15
y11 (2+) 12.9 11.7 6.8 24.4 11.3 7.8 27.6 16.3 10.3 15 24 15
y5 (1+) 49.6 35.7 34.7 61.6 33 42.3 79.1 48.6 54.7 15 24 15
y7 (1+) 22.5 13.1 10.9 23.6 14.4 11.5 32.6 19.5 15.8 15 24 15
sum 12.3 11.6 7.1 23.5 11.1 7.9 26.5 16.1 10.6 15 24 15

Additional Resources and Comments