University at Buffalo - The State University of New York
Skip to Content
PubMed Central, Figure 4: Oncogene. 2016 Nov 3; 35(44): 5781–5794. Published online 2016 May 2. doi:  10.1038/onc.2016.112
Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
PMC full text:
Oncogene. Author manuscript; available in PMC 2016 Nov 7.
Published in final edited form as:
Oncogene. 2016 Nov 3; 35(44): 5781–5794.
Published online 2016 May 2. doi:  10.1038/onc.2016.112

Figure 4

An external file that holds a picture, illustration, etc.
Object name is nihms-753191-f0004.jpg
cREL, ΔNp63α, and TAp73 modulate transcriptional regulation and differential gene expression

(A) UM-SCC46 cells were transfected with luciferase reporter plasmids containing specific TP53, AP-1, or NF-κB REs (upper panels), or the promoter sequences of CDKN1A (p21) (TP53, TP63 REs), SERPINE1 (AP-1 REs) or IL-6 (AP-1 and NF-κB REs; lower panels). Overexpression of cREL, ΔNp63α, or TAp73α was induced by TNF-α treatment (20 ng/ml) for 48 h. IL-6 binding site-specific point mutant promoter constructs included the deletion mutation of NF-κB or AP-1 binding sites without TNF-α (bottom right panel). The relative reporter activity was normalized to the corresponding β-gal activity and/or compared with the control vectors. Blue, untreated; red, TNF-α treated, except in IL-6 reporter assay (bottom right panel). In IL-6 reporter assay without TNF-α treatment, blue: reporter with full length IL-6 promoter; red: IL-6 promoter with the NF-κB binding motif deleted; green: IL-6 promoter with the AP-1 binding motif deleted. (B) The newly identified target genes were validated by q-RT-PCR 48 h after cREL, ΔNp63α, or TAp73α overexpression under TNF-α treatment (20 ng/ml). Blue, untreated; red, TNF-α treated. The data are presented as the mean ± SD of three replicates from one representative experiment. Statistical significance was calculated using a two-tailed Student's T-Test, p<0.05. * indicates the statistical significance when comparing the conditions with overexpressed plasmids versus control plasmid. # indicates the statistical significance when comparing untreated versus TNF-α treated condition.

Images in this article

  • Figure 1
  • Figure 2
  • Figure 3
  • Figure 4
  • Figure 5
  • Figure 6
  • Figure 7
  • Figure 8
Click on the image to see a larger version.