Reaching Others
SEARCH SDM

Home
 
About Oral Biology
 
Academics/Training
 
Research
 
Facilities and Resources
 
Admissions
 
Alumni
 
Support the School
 About Oral BiologyNew Home     November 1, 2014  

Stefan Ruhl  PhD, DDS

Department of Oral Biology

Associate Professor, DDS, PhD

Specialty/Research Focus

Operative Dentistry; Oral Biology; Pediatric Dentistry; Periodontics

Faculty Research Profile

 
top-left cornertop-right corner
Professional Summary:

Research in my laboratory encompasses the general area of oral infection and immunity with a major focus on adhesin-mediated interactions of oral bacteria with host salivary or cellular receptors. We investigate the proteins in saliva that are recognized by lectin-like microbial adhesins. It is our long-term goal to better understand the modulating role of salivary glycoproteins in supporting tissue tropism of a benign commensal oral microflora to the human oral cavity and in host defense against pathogenic microorganisms. A more detailed description of our current research activities can be found on the following website:

Education and Training:
  • Professor (C3), Operative Dentistry and Periodontology, Universität Regensburg (2004)
  • Privatdozent, venia legendi, Operative Dentistry and Periodontology, Universität Regensburg (2000)
  • PhD, Dr. med. dent., Georg-August-Universität (1988)
  • DDS, Dentistry, Georg-August-Universität (1984)
Employment:
  • Associate Professor, Oral Biology, University at Buffalo (2011-present)
  • Adjunct Associate Professor, Microbiology and Immunology, University at Buffalo (2011-present)
  • Assistant Professor, Oral Biology, University at Buffalo (2007-present)
  • Adjunct Assistant Professor, Microbiology and Immunology, University at Buffalo (2008-2010)
  • Professor (C3) Germany, Department of Operative Dentistry and Periodontology, University of Regensburg (2004-2007)
  • Associate Professor (C2) Germany, Department of Operative Dentistry and Periodontology, Universität Regensburg (2000-2004)
  • Assistant Professor (C1) Germany, Operative Dentistry and Periodontology, University of Regensburg (1995-2000)
  • Laboratory of Microbial Ecology, National Institute of Dental Research, NIH (1991-1994)
  • Division of Cytokine Biology, Center for Biologics Evaluation and Research, FDA (1989-1991)
  • Laboratory of Immunology, National Institute of Dental Research, NIH (1988-1989)
  • Private practice in Germany (1986-1988)
  • Doctoral Candidate, Institute of Medical Microbiology and Immunology, Georg-August-University, Göttingen, Germany (1985-1986)
  • Hochgebirgszug, Gebirgsjäger, Bad Reichenhall, German Military (1978-1979)
See All (13 Total) >
Awards and Honors:
  • Omicron Kappa Upsilon, National Dental Honor Society (2014)
  • UB-SDM Society for the Advancement of Dental Research Award (2011)
  • WALKHOFF Award (2007)
  • WRIGLEY Award (2007)
  • Dentsply Award (2005)
  • Dentsply Award (1999)
  • CORSODYL Research Award (1998)
  • Poster Award, 17th International Lectin Conference (1997)
  • Poster Award, German Society for Operative Dentistry (1996)
  • Postdoctoral Fellowship, German Research Foundation (DFG) (1988)
  • Postgraduate Fellowship (GradFöG), Universität Göttingen (1985)

Research Expertise:
  • Glycobiology of microbial adhesion
  • Bacterial adhesion to biomaterials
  • Salivary proteomics
Grants and Sponsored Research:
  • April 2014-April 2015
    The functional & biomedical implications of the unusual genetic variation in the salivary MUC7 protein
    UB-IMPACT
    Role: Co-Principal Investigator
  • July 2013-July 2015
    Supplement to: Microbial recognition of sialic acid diversity in the salivary proteome
    NIH/NIDCR
    Role: Principal Investigator
  • July 2011-June 2012
    Saliva Spectral Analysis for New Fertility Diagnosis
    NYSTAR / UB-CAT
    Role: Principal Investigator
  • July 2010-June 2015
    Microbial recognition of sialic acid diversity in the salivary proteome
    NIH
    Role: Principal Investigator
  • July 2007-July 2009
    Charged dendrimers as an antibacterial coating for biomaterial surfaces
    German Research Foundation (DFG)
    Role: Co-Principal Investigator
  • February 2006-July 2007
    Identification of receptors in human saliva for the adhesins of Actinomyces viscosus T14V
    German Society for Dental and Cranio-mandibular Science (DGZMK)
    Role: Principal Investigator
  • January 2002-December 2005
    Interactions of Helicobacter pylori with salivary glycoproteins
    German Research Foundation (DFG)
    Role: Principal Investigator
  • January 1999-December 2000
    Salivary proteins and glycoproteins in children with nursing bottle caries
    German Research Foundation (DFG)
    Role: Principal Investigator
  • June 1998-June 1998
    Travel grant
    German Society for Dental and Cranio-mandibular Society (DGZMK)
    Role: Principal Investigator
  • January 1997-December 1997
    ReForM-A
    University of Regensburg
    Role: Principal Investigator
  • March 1996-March 1996
    Travel grant
    German Research Foundation (DFG)
    Role: Principal Investigator
  • July 1988-June 1990
    Immunologic, microbiologic aspects of the caries process / periodontal diseases
    German Research Foundation (DFG)
    Role: Principal Investigator
  • July 1985-April 1986
    Characterization of human dendritic cells and their functions
    State of Niedersachsen
    Role: Principal Investigator
See All (13 Total) >

Journal Articles:
See All (39 Total) >

Abstracts:
  • Hei S, Choi K, Kazim LA, Haase EM, Reddy M, Scannapieco FA, and Ruhl SH. Binding between human salivary components and Staphylococcus aureus. J Dent Res. 2012.
  • Eidt A, Lee DH, Heo SM, Haase EM, Reischl U, Cisar JO, Scannapieco FA, Ruhl S. Coadhesion Partners for Helicobacter pylori Among Oral Biofilm Microbiota. J Dent Res. 2010; 89.
  • Ruhl S, Eidt A, Reischl U, Cisar JO. Coadhesion Partners for Actinomyces naeslundii from Dental Biofilm Microbiota. J Dent Res. 2010; 89.
  • Heo, S-M., Choi, K.S., Kazam, A.L., Reddy, M.S., Haase, E.M., Scannapieco, F.A., and Ruhl, S.H.. Characterization of human salivary components associated with Staphylococcus aureus. J Dent Res. 2010.
  • Ruhl S. Helicobacter pylori in the Oral Cavity and the Role of Saliva. J Dent Res. 2009; 88.
  • Palmer R, Ruhl S, Kolenbrander PE, Cisar JO. Veillonellae selectively recognize streptococcal Galb1-3GalNAc-containing receptor polysaccharides. J. Dent. Res.. 2007.
  • Muller R, Hiller KA, Schmalz G, Ruhl S. Chemiluminescence-based method for quantification of adsorbed proteins on material surfaces. J. Dent. Res.. 2007.
  • Walz A, Stuehler K, Wattenberg A, Hawranke E, Meyer HE, Schmalz G, Blueggel M, Ruhl S. Consistency and variance in human saliva 2-D proteome. J Dent Res. 2006; 85(A).
  • Ruhl S, Ganga-Raju P, Langenfelder S, Horl D, Lehn N, Schmalz G, Hiller K-A, Durchschlag H. Sterlization of human saliva by irradiation or filtration. J Dent Res. 2006; 85(A).
  • Hiller K-A, Dorner S, Kaes C, Schmalz G, Ruhl S. Quantification of adsorbed salivary proteins on dental biomaterial surfaces. J Dent Res. 2006; 85(A).
  • Muller R, Ruhl S, Hiller K-A, Schmalz G, Schweikl H. Fibronectin enhances cell adhesion on a hydrophobic fluorocarbon surface. Biomaterialien. 2006.
  • Walz A, Ruhl S. Rezeptoren fur H. pylori im Speichel. Dtsch Zahnarztl. 2005; Z(D).
  • Engel K, Aspholm M, Walz A, Ruhl S, Faller G, Haas R, Boren T, Odenbreit S. Interrelations between AlpAB-,BabA- and SabA-dependent adherence activities of Helicobacter pylori. Biospektrum. 2005; GIV.
  • Walz A, Wattenberg A, Schmalz G, Blueggel M, Ruhl S. Proline-rich proteins as major part of the human saliva proteome. J Dent Res. 2005; 84(A).
  • Ruhl S, Eidt A, Cisar JO. A bacteria overlay method for detecting specific interbacterial adhesion. J Dent Res. 2005; 84(A).
  • Muller R, Ruhl S, Englert C, Nerlich M, Groger G, Hiller K-A, Schmalz G, Schweikl H. Different adhesion behavior of bacteria and eukaryotic cells on model surfaces. Biomaterialien. 2005; 6(3).
  • Ruhl S. Adhasion von Bakterien auf Oberflachen zahnarztlicher Werkstoffe. Dtsch Zahnarztl. 2005; Z(A).
  • Muller R, Thalmaier U, Schmalz G, Ruhl S. Bacterial adhesion to surfaces of defined polarity in relation to previous protein adsorption. Eur Cell Mater. 2004; 7(1).
  • Burgers R, Schneider-Brachert W, Reischl U, Schmalz G, Hiller K-A, Ruhl S. Helicobacter pylori in human oral cavity and stomach. J Dent Res. 2004; 83(B).
  • Walz A, Odenbreit S, Schmalz G, Ruhl S. Characterisation of binding specificities of Helicobacter pylori adhesins by glycoconjugate arrays. Glycoconj J. 2004; 21.
  • Ruhl S, Hamberger S, Betz R, Sukkar T, Schmalz G, Seymour RA, Hiller K-A, Thomason JM. Cytokines and growth factors in saliva of patients with cyclosporin-induced gingival overgrowth. J Dent Res. 2003; 82(B).
  • Ruhl S, Schmalz G, Seymour RA, Betz R, Hamberger S, Hiller K-A, Thomason JM. Salivary proteins and glycoproteins in patients with cyclosporin-induced gingival overgrowth. J Dent Res. 2003; 82(C).
  • Ruhl S, Rayment SA, Schmalz G, Troxler RF. Expression of salivary mucins during the first year of infancy. J Dent Res. 2002; 81(A).
  • Ruhl S, Heep M, Schneider-Brachert W, Schmalz G. Adhasion von Helicobacter pylori an Speichelproteinen. Dtsch Zahnarztl Z. 2002(D).
  • Ruhl S, Schmalz G, Hamberger S, Thomason JM, Hiller K-A, Seymour RA. Salivary cytokines in patients with cyclosporin-induced gingival overgrowth. J Dent Res. 2000; 79.
  • Ruhl S, Betz R, Hamberger S, Schmalz G. Influence of storage and centrifugation on the electrophoretic pattern of salivary proteins. J Dent Res. 2000; 79.
  • Heep M, Ruhl S, Wehrl W, Senzenberger S, Lehn N, Schmalz G. Adhesion of different strains of H. pylori to salivary glycoproteins. Gut 47. 2000; 47(A).
  • Ruhl S, Schmalz G. Salivary proteins and glycoproteins during the first year of infancy. J Dent Res. 1999; 78.
  • Ruhl S, Schmalz G. Analysis of donor-specific salivary glycoprotein profiles by lectin blotting. J Dent Res. 1998; 77.
  • Ruhl S, Sandberg AL, Cisar JO. Lectin-mediated binding of streptococci and actinomyces to granulocyte glycoprotein receptors. J Dent Res. 1997; 76.
  • Ruhl S, Sandberg AL, Cisar JO. Lectin-mediated binding of streptococci and actinomyces to leukosialin and leukocyte common antigen on granulocytes. Eur J Cell Biol. 1997; 74(46).
  • Ruhl S, Sandberg AL, Cisar JO. Salivary proteins and glycoproteins recognized by actinomyces and streptococcal adhesins. J Dent Res. 1996; 75.
  • Ruhl S, Cisar JO, Cole M, Sandberg AL. Recognition of IgA by oral bacterial lectins. Adv Dent Res. 1996; 11.
  • Ruhl S, Sandberg AL, Cole MF, Cisar JO. Surface-associated IgA1 as an attachment receptor for oral bacterial lectins. Immunobiol. 1996; 196.
  • Takahashi Y, Cisar JO, Ruhl S, Sandberg AL. A Streptococcus gordonii DL1 antigen with sialic acid lectin reactivity. J Dent Res. 1995; 74.
  • Ruhl S, Sandberg AL, Cisar JO. Leukosialin (CD43), a granulocyte receptor for streptococcal and actinomyces lectins. J Dent Res. 1994; 73.
  • Ruhl S, Sandberg AL, Cisar JO. Binding of actinomyces and streptococci to HL-60 cells during differentiation towards granulocytes. J Dent Res. 1993; 72.
  • Ruhl S, Cohen B, Pluznik DH. Transcription is a major mechanism regulating the synergistic action of IL-6 and IL-1 on interleukin-2 receptor expression during myeloid differentiation. J Cell Biochem. 1991; 15(F).
  • Ruhl S, Feldman GM, Finbloom DS, Pluznik DH. Regulation of interleukin-4 receptor expression on primitive and mature myeloid cells by the hematopoietic growth factor interleukin-6. Exp Hematol. 1991; 19.
  • Ruhl S, Bickel M, Pluznik DH. Differential regulation of leukemia inhibitory factor-and interleukin-6-induced expression of surface markers in differentiating myeloid cells. J Cell Biochem Suppl. 1990; 14(B).
  • Ruhl S, Pluznik DH. Early and late events during myeloid differentiation can be distinguished by use of interleukin-6 and interferon-gamma. Exp Hematol. 1990; 18.
  • Ruhl S, Schwabe M, Pluznik DH. Interleukin-1 augments interleukin-2 receptors on differentiating macrophages. Lymphokine Res. 1990; 9.
  • Feldman GM, Ruhl S, Pluznik DH, Finbloom DS, Bickel M. Mechanism of interleukin-4 receptor regulation during macrophage activation by IL-6. Lymphokine Res. 1990; 9.
  • Ruhl S, Begley CG, Bickel M, Pluznik DH. Interleukin-2 receptor (IL2R) on murine myeloid leukemia cells is induced by leukemia inhibitory factor (LIF) and interleukin-6 (IL6) and represents an early marker of myeloid differentiation. Exp Hematol. 1989; 17.
  • Dieffenbach CW, Dveksler G, Ruhl S, Bickel M, Pluznik. Interferon and 2‘5‘A synthetase mRNA levels in murine monocytic leukemia cells (M1) after induction of differentiation. J Interferon Res. 1989; 9.
  • Ruhl S, Begley CG, Bickel M, Pluznik DH. Transient expression of interleukin-2 receptors during early stages of interleukin-6 and leukemia inhibitory factor induced murine myeloid cell differentiation. Cytokine. 1989; 1.
  • Peters JH, Ruhl S, Friedrichs D, Saur T. Veiled accessory (dendritic cells) deduced from monocytes. Immunobiol. 1987; 175.
See All (47 Total) >
Books and Book Chapters:
Professional Memberships:
  • Society for Glycobiology (2008)
  • Consortium for Functional Glycomics (2008)
  • American Association for Dental Research (AADR) (2008)
  • German Society for Biochemistry and Molecular Biology (GBM) • Studygroup Glycobiology (1997)
  • International Association of Dental Research (IADR) , Salivary Research Group, Microbiology and Immunology Interest Group (1995)
  • German Society of Dental, Oral, and Craniomandibular Sciences (DGZMK) • German Society for Operative Dentistry (DGZ) • German Society for Pediatric Dentistry and Prophylaxis (DGK) • Studygroup for Basic Science (AfG) (1995)
  • German Society for Immunology (1995)
Presentations:
  • The Salivary Proteome - Oral Microbiome Interface, 50th Anniversary, UB Oral Biology Graduate Program (2014)
  • Human Lineage Specific Traits in the Salivary Proteome, IADR General Session & Exhibition, IADR (2014)
  • Keynote: Studies on the Salivary Proteome - Oral Microbiome Interface, 43rd Annual Meeting & Exhibition of the AADR, AADR (2014)
  • The Salivary Proteome - Oral Microbiome Interface, Invited Seminar Lecture, University of Minnesota, School of Dentistry (2013)
  • How I Became a Research Scientist, UB BioSTEM Lecture Series - CLIMB UP (2013)
  • The Importance of Saliva for a Healthy Mouth, UB Discovery Seminar - Oral Biology, University at Buffalo (2013)
  • How does the Salivary Proteome Modulate Bacterial Adhesion?, Bagel Talk, University of Rochester, Center for Oral Biology, University of Rochester (2012)
  • The Importance of Saliva for a Healthy Mouth - from a Glycobiologist's Perspective, SACNAS National Conference, SACNAS (2012)
  • New Aspects in Cariology, UB SDM Back-to-School Conference (2012)
  • Binding between Human Salivary Components and Staphylococcus aureus, 41st Annual Meeting of the AADR, AADR (2012)
  • Control of Oral Biofilm Formation on Biomaterials, UB Department of Chemistry (2012)
  • Control of Oral Biofilm Formation on Biomaterials, UB Integrated Nanoscience Workshop (2012)
  • The Importance of Saliva for the Dentist, UB-SDM Continuing Education (2011)
  • How Saliva Mediates Bacterial Adhesion, The New Jersey Dental School (2011)
  • It's in the Spit:: The Diagnostic Potential of Saliva, Buffalo Niagara Dental Meeting (2009)
  • The Diagnostic Potential of Saliva, Continuing Dental Education, UB School of Dental Medicine (2008)
  • A proteomics-based search for bacterial recognition sites in human saliva, Invited lecture, Department of Microbiology and Immunology, University at Buffalo (2008)
  • A proteomics-based search for bacterial recognition sites in human saliva, Rochester Oral Biology Research Conference (2008)
  • Bakterielle Erkennungsstrukturen im menschlichen Speichelproteom, Invited lecture, Medical Faculty, University of Gottingen, Germany, University of Gottingen (2007)
  • Das menschliche Speichelproteom als Grundlage fur zukunftige Diagnostik, Deutscher Zahnarztetag 2007 (2007)
  • Kinderzahnheilkunde und Alterszahnmedizin - Ein Gegensatz, Invited lecture, Medical Faculty, University of Gottingen, Germany, University of Gottingen (2007)
  • Sticky spots in spit - a proteomics-based search for bacterial recognition sites in human saliva, Invited Lecture, Umea University, Sweden, Umea University (2006)
  • Adsorption of proteins and attachment of bacteria to biomaterial surfaces, International Symposium Material - Tissue Interactions (2006)
  • The importance of saliva, Visit of the Academia Conservativa Italiana, University of Regensburg (2006)
  • Wissenswertes fur den Zahnarzt uber Speichel, Workshop: Speichel - "ein ganz besonderer Saft" (2006)
  • Spot Spitting and Spit Spotting: A Proteomics-Based Search for Functional Aspects in Saliva, Invited lecture, University of Buffalo (2006)
  • Adsorption von Proteinen und Adhasion von Bakterien auf Modelloberflachen und klinischen Werkstoffen, Medizinische Fakultat, Universitat Regensburg (2005)
  • Adhasion von Bakterien auf Oberflachen zahnarztlicher Werkstoffe, Symposium der Arbeitsgemeinschaft fur Grundlagenforschung in der DGZMK (2005)
  • Bedeutung des Speichels fur ein gesundes Mundmillieu, Klinischer Arbeitskreis im Qualitatszirkel des ZBV Oberpfalz (2005)
  • Bestandteile des menschlichen Speichels und ihre Bedeutung, Institut fur Physikalische und Theroetische Chemie, Universitat Regensburg (2005)
  • Speichel-'ein ganz besonderer Saft, Medizinische Fakultat, Universitat Leipzig (2005)
  • Der Speichel als diagnostische Flussigkeit, Medizinische Facultat, Universitat Regensburg (2003)
  • Speichel als diagnostische Flussigkeit, 2.Regionalkonferenz des Vereins zur Forderung der wissenschaftlichen Zahnheilkunde in Bayern, Kloster Seeon (2003)
  • Grundlagen und Stategien zahnarztlicher Prophylaxe, Klinischer Arbeitskreis im Qualitatszirkel des ZBV Oberpfalz (2003)
  • Adhasin-vermittelte Interaktionen oraler Midroorganismen mit Strukturen der Mundhohle, Institut fur Medizinische Mikrobiologie, Friedrich-Schiller-Universitat Jena (2003)
  • Prophylaxe, mehr als nur Einfarben. Individualprophylaxe-Theorie und Praxis, 43. Bayerischer Zahnarztetag (2002)
  • Moderne Prophlyaxe: Moglichkeiten, Chancen, Grenzen. Bleichung von Zahnen. Kinderzahnheilkunde:Endodontologie, Zahnarztlicher Fortbildungskreis, Rottal-Inn e.V. (2002)
  • Adhasin-vermittelte Interaktionen von oralen Bakterien mit Strukturen der Mundhohle, Mikrobiologisch-Immunologisches Kolloquium, Univeritat Ulm (2002)
  • Immunologie der Mundhohle, Vorlesung Immunologie fur Zahnmediziner, Universitat Ulm (2002)
  • Der diagnostische Stellenwert des Speichels fur die Kinderzahnheilkunde, Medizinische Fakultat, Christian-Albrechts-Universitat zu Kiel (2002)
  • Proteine und Glykoproteine im Speichel: Funktionen und mogliche klinische Bedeutung, Zahn-, Mund- und Kieferklinik, Ludwig-Maximilians-Universitat Munchen (2001)
  • Der Speichel als eine diagnostische Flussigkeit, Zentrum fur Zahn-, Mund- und Kieferheilkunde, Rheinische Friedrich-Wilhelms-Universitat Bonn (2001)
  • Bleichung von Zahnen: Moglichkeiten und Grenzen, Klinischer Arbeitskreis im Qualitatszirkel des ZBV Oberpfalz (2001)
  • Moderne Prophylaxe: Moglichkeiten, Chancen, Grenzen, 15. Oberpfalzer Zahnarztetag (2001)
  • Adhasin-vermittelte Interaktionen oraler Mikroorganismen mit Strukturen der Mundhohle, Zentrum fur Biomaterialien an der Universitat Regensburg (2001)
  • Adhasin-vermittelte Interaktionen von oralen Bakterien mit Strukturen der Mundhohle, Medizinische Fakultat, Rheinisch-Westfalische Technische Hochschule (2001)
  • Salivary Proteins and Glycoproteins: Interactions with the Oral Microbial Flora and Possible Clinical Relevance, Oral Infection and Community Branch, National Institute of Dental and Craniofacial Research, NIH (2000)
  • Kinderbehandlung: Milchzahnendodontie, Klinischer Arbeitskreis im Qualitatszirkel des ZBV Oberpfalz (1998)
  • Proteine und Glykoproteine im Speichel: Funktion und klinische Relevanz, 39. Bayerischer Zahnarztetag 1998 (1998)
  • Role of Sialic Acids in the Adhesion of Oral Bacteria, Sialinsaure-Workshop, Biochemisches Institute, Christian-Albrechts-Universitat zu Kiel (1997)
  • Salivary and Cell Surface Glycoprotein Receptors for Oral Bacterial Lectins as Mediators of Host-Microbial Interactions, ERGOB Meeting 1996 (1996)
  • Nomenklatur, Atiologie und Pthogenese der marginalen Parodontopathien, 3. Zliner Zhnarztetag (1996)
  • Lektin-vermittelte Interaktion von oralen Bakterien mit granulozytaren Glykoproteinrezeptoren, Mikroibiologisch-Immunologisches Kolloquium, Hygiene-Institut der Universitat Gottingen (1995)
  • Bacterial Lectin-Receptors on Human Granulocytes, The Procter & Gamble Company, Worldwide Clinical Investigation - Oral (1994)
  • Polymorphonuclear Leukocyte Receptors for Oral Bacterial Lectins, Research Center in Oral Biology, University of Pennsylvania (1994)
  • Leukosialin: A Granulocyte Receptor for Streptococcal and Actinomyces Lectins, NIH-Interinstitute Glycobiology Interest Group, National Institutes of Health (1994)
  • Der Interleukin-2 Rezeptor, Klinik fur Zahnarztliche Prothetik, Universitat Bern (1991)
  • Regulation of IL-2 Receptor Expression on Differentiating Macrophages, Forsyth Dental Center (1991)
  • Regulation von Interleukin-2 Receptoren vahrend der Makrophagen-Differnzierung, Mikrobiologisch-Immunologisches Kolloquium, Hygiene-Institut der Universitat Gottingen (1991)
  • The Interleukin-2 Receptor on Haematopoietic Cells, Laboratory of Immunology, National Institute of Dental Research, NIH (1991)
  • Der Interleukin-2 Rezeptor und seine Rolle im Prozeb der Differenzierung myeloider Zellen, Poliklinik fur Zahnarztliche Chirurgie U. Parodontologie, Eberhard-Karls-Universitat Tubingen (1989)
See All (61 Total) >
Service Activities:
  • School of Dental Medicine Student Research and Honors Committee; Co-Chair (2008)
  • School of Dental Medicine Admissions Committee; Member (2007-2013)

  • Past President, IADR Salivary Research Group; Past President (2014-2015)
  • IADR Keynote Address; Salivary Gland Organogenesis, Engineering and Evolution; Chair (2014-2014)
  • IADR Symposium: Current Updates on Dry Mouth Diagnosis and Management: Where Are We?; Chair (2014-2014)
  • Organizer and Chair: IADR Salivary Research Group Business Meeting, Cape Town, SA; Chair (2014-2014)
  • Organizer: IADR Symposium "Novel Approaches to Functional Regeneration of the Salivary Gland"; Coordinator (2013-2013)
  • Resarch Panel, UB Pre-Health Symposium; Panel Member (2013-2013)
  • IADR Symposium: Novel Approaches to Functional Regeneration of the Salivary Gland; Chair (2013-2013)
  • IADR/AADR Salivary Research Group; President (2013-2014)
  • Research Panel, UB Prehealth Symposium; Panel Member (2013-2013)
  • AADR Saliva in Health and Disease; Chair (2012-2012)
  • IADR/AADR Salivary Research Group; Vice President (2012-2013)
  • AADR Oral Biology (Seq. #38); Chair (2010-2010)
  • AADR Fellowship Committee; Member (2010)
  • AADR/IADR Salivary Research Group; Committee Member (2010-2012)
  • Organizer of the Department's Seminar Series in Oral Biology Seminar; Coordinator (2009-2012)
  • Salivary Research, Keynote Address and Normal Homeostasis; Co-Chair (2008-2008)
  • Saliva - Biomarkers / Structure and Function; Rochester Oral Biology Research Conference; Co-Chair (2008-2008)
  • Symposium "Saliva - Diagnostic Medium of the Future"; Annual meeting of the German Dental Association, November 22, Dusseldorf, Germany; Chair (2007-2008)
  • Study Group of Basic Sciences; AfG, DGZMK, Mainz, Germany; Chair (2006-2006)
  • Adherence/Adhesion (Seqq. #301.0); 83rd General Session and Exhibition of the International, American and Canadian Associations for Dental Research, Baltimore, MD; Chair (2005-2005)
  • Study Group of Basic Sciences; AfG, DGZMK, Mainz, Germany; Chair (2005-2005)
  • Editorial Board of the Journal of Dental Research; Editorial Board Member (2005-2007)
  • Committee for Therapeutical Drugs; Member (2005-2007)
  • Training for University Teachers in Pediatric Dentistry; Germany; Chair (2004-2004)
  • Gingival Overgrowth (Seq. #254); 81st General Session and Exhibition of the International Association for Dental Research, Goteborg, Sweden; Chair (2003-2003)
  • Caries Risk Management; ConsEuro, Munich, Germany; Chair (2003-2003)
  • Study Group of Basic Sciences; Scientific Program, AfG, DFZMK, Hannover, Germany; Chair (2002-2002)
  • 6th European Symposium on Saliva,; Egmond aan Zee, The Netherlands; Chair (2002-2002)
  • Mechanism of Secretion/Protein Expression (Seq. #45),; 80th General Session and Exhibition of the International Association for Dental Research, San Diego, CA; Chair (2002-2002)
  • Oral Biology of the Child, 8; Jahrestagung der Gesellschaft fur Kinderzahnheilkunde und primarprophyaxe (GKP) in der DGZMK; Chair (2001-2001)
  • Board of Examiners; Final exam for D.D.S. degree; Board Member (2001-2007)
  • Salivary Proteins/Molecules (Seq. #45); 77th General Session and Exhibition of the International Association for Dental Research, Vancouver, Canada; Chair (1999-1999)
  • Microbiology/Immunology I (Seq. #3); 3rd CED/NOF Joint Meeting, Berlin, Germany; Chair (1996-1996)
  • German Research Foundation (DFG); Two article reviews; External Reviewer (1995-2007)
  • Review Activities; Reviewer for the Following Scientific Journals: Clinical Oral Investigation Deutsche Zahnarztliche Zeitschrift Journal of Dental Research European Journal of Oral Sciences Archives of Oral Biology Oral Diseases Caries Research Anatomia Histologia Embryologia J. of Veterinary Medicine Proteomics Planta Medica-Natural Products and Medicinal Plant Research Journal of Pharmacological Sciences Oral Biosciences and Medicine Biomacromolecules Drug Discovery Today Immunological Investigations Journal of Anatomy Journal of Nanoscience and Nanotechnology Journal of Proteome Research; Reviewer (1995)
See All (37 Total) >

bottom-left cornerbottom-right corner

Contact Information

Department of Oral Biology
University at Buffalo
Foster Hall, Room 213A
Buffalo, NY 14214-3092
Phone: (716) 829-6073
Fax: (716) 829-3942
Email: shruhl@buffalo.edu


Log in to Update Your Profile

Ruhl, Stefan DDS, PhD Associate Professor, Department of Oral Biology

Address:
213A Foster Hall
Buffalo, NY 14214
(716)(716) 829-6073
shruhl@buffalo.edu

Faculty Profile

2011 Associate Professor, Oral Biology
Adjunct Associate Professor, Department of Microbiology and Immunology
2008 Adjunct Assistant Professor, Department of Microbiology and Immunology
2007 Assistant Professor, Department of Oral Biology, School of Dental Medicine
2004-07 C3-Professor, Universität Regensburg, Germany
2000-04 Privatdozent, venia legendi, Universität Regensburg
1995-2000 Assistant Professor, Department of Operative Dentistry and Periodontology, Universität Regensburg
1991-94 Visiting Associate, Laboratory of Microbial Ecology, NIDR, NIH, Bethesda, MD
1989-91 Visiting Associate, Division of Cytokine Biology, CBER, FDA, Bethesda, MD
1988-89 Guest Researcher, Laboratory of Immunology, NIDR, NIH, Bethesda, MD
1986-88 Dentist, Private practice, Germany
1984-86 Doctoral Candidate, Department of Immunology, Institute of Medical Microbiology and Immunology, Georg-August-Universität, Göttingen
1979-84 Dental School, Georg-August-Universität, Göttingen, Germany

Selected Affiliations:
International Association for Dental Research (IADR)
Society for Glycobiology
Consortium for Functional Glycomics
Witebsky Center for Microbial Pathogenesis & Immunology
German Society for Dental and Craniofacial Research (DGZMK)
German Society for Immunology (DGI)
German Society for Biochemistry and Molecular Biology (GBM), Studygroup Glycobiology

Research Profile

Dr. Ruhl's scientific interest encompasses the general area of oral infection and immunity with major emphasis on the adhesin-mediated interactions of oral bacteria with host salivary or cellular receptors.

Saliva is acknowledged as a major contributor to oral health. Among many other protective functions in the human mouth, saliva helps to maintain an ecological balance within the diverse oral biofilm microbiota through fostering colonization by harmless commensal bacteria that may create a microclimate beneficial for oral health. Already the initial events of bacterial colonization are dependent on saliva, in that certain salivary proteins and glycoproteins, once adsorbed to the oral mineralized or tissue surfaces, serve as adhesion substrates for early colonizing bacteria such as oral actinomyces and viridans streptococci. Many of these early colonizers possess surface adhesins that recognize and bind to cognate peptide or carbohydrate motifs on salivary proteins.



Although the specificities of some of the bacterial adhesins are well characterized, the range of salivary proteins, which serve as receptors for these adhesins, is not fully explored. Past studies in this group have identified salivary proteins that are recognized by the PRP-binding and lectin-like Gal/GalNAc-specific adhesins of oral actinomyces as well as by PRP-binding and sialic acid-specific adhesins of oral viridans streptococci (Ruhl et al. 2004).



Current studies in this laboratory now utilize proteomics methods for a detailed investigation of the full range of salivary proteins and their function as receptors mediating bacterial attachment. As a basis for this goal, proteome maps have been established for whole mixed saliva as well as for the isolated secretions of the major salivary gland (Walz et al., 2006).



Currently, the interaction of salivary proteins with pathogenic bacteria not commonly associated with the oral cavity is being investigated. As an example, the stomach pathogen Helicobacter pylori was selected. This bacterium has recently been found in oral biofilms and was proposed to possess an ecological niche in the oral cavity, which – if true – may have important implications for reinfection of the stomach or transmission between individuals (Bürgers et al., 2008). In a collaborative project with Thomas Borén, Umeå University, Sweden, and Stefan Odenbreit, Max-von-Pettenkofer-Institute, Munich, Germany, isogenetic knock-out mutants of H. pylori, that are deficient in either the sialic acid-binding adhesin (SabA) or in the Lewis-b-binding adhesin (BabA), were used as probes to identify potential salivary receptors that mediate binding of these bacteria (Walz et al. 2005; Walz et al. 2009). Findings resulting from this investigation could help to better understand the role of saliva in the pathogenesis of H. pylori infection including its ability to colonize the oral cavity as an ecological niche.



To obtain a reliable basis for clinical investigations, it is first necessary to test intra- and interindividual as well as age-dependent variations in the composition of salivary proteins. In this context, studies were performed to investigate protein and cytokine composition in individuals with severe gingival disease (Ruhl et al., 2004) as well as longitudinal changes in salivary protein composition during infant development (Ruhl et al., 2005) or differences in salivary protein profiles in children with early childhood caries (Zehetbauer et al., 2009) with the aim to discover clinically relevant indicators among salivary proteins.

In a collaborative project with the Institute of Physical and Theoretical Chemistry, the Department of Operative Dentistry, University of Regensburg, and the Dental School, University of Tübingen, Germany, the influence of surface chemistry of certain biomaterials including those used in the oral cavity on protein adsorption and bacterial adhesion is being investigated (Müller et al., 2006, 2007). Findings from these latter studies may allow to develop strategies to modify biomaterial surfaces in order to minimize bacterial adhesion to biomaterials not only in the oral cavity but also in other regions of the human body.

Representative Publications Search Publications in MEDLINE

Heo SM, Choi KS, Kazim LA, Reddy MS, Haase EM, Scannapieco FA, Ruhl S (2013) Host defense proteins derived from human saliva bind to Staphylococcus aureus. Infect. Immun. 81: 1364-1373

Ruhl S (2012) The scientific exploration of saliva in the post-proteomic era: from database back to basic function. Expert Rev Proteomics 9: 85-96

Katzur V, Eichler M, Deigele E, Stage C, Karageorgiev P, Geis-Gerstorfer J, Schmalz G, Ruhl S, Rupp F, Müller R (2012) Surface-immobilized PAMAM-dendrimers modified with cationic or anionic terminal functions: physicochemical surface properties and conformational changes after application of liquid interface stress. J. Colloid Interface Sci 366: 179-90.

Eichler M, Katzur V, Scheideler Lutz, Haupt M, Geis-Gerstorfer J, Schmalz G, Ruhl S, Müller R, Rupp F (2011) The impact of dendrimer-grafted surface modifications to model silicon surfaces on protein adsorption and bacterial adhesion. Biomaterials 32: 9168-9179.

Ruhl S, Berlenbach P, Langenfelder S, Hörl D, Lehn N, Hiller K-A, Schmalz G, Durchschlag H (2011) Integrity of proteins in human saliva after sterilization by gamma irradiation. Appl. Environ. Microbiol. 77: 749-755.

Odenbreit S, Swoboda K, Barwig I, Ruhl S, Borén T, Koletzko S, Haas R (2009) Outer membrane protein (OMP) expression profile in Helicobacter pylori clinical isolates. Infect. Immun. 77: 3782-3790.

Müller R, Eidt A, Hiller K-A, Katzur V, Subat M, Schweikl H, Imazato S, Ruhl S, Schmalz G (2009) Influences of protein films on antibacterial or bacteria-repellent surface coatings in a model system using silicon wafers. Biomaterials 30: 4921-4929.

Zehetbauer S, Wojahn T, Hiller K-A, Schmalz G, Ruhl S (2009) Resemblance of salivary protein profiles in children with early childhood caries. Eur. J. Oral Sci. 117: 369-373.

Walz A, Odenbreit S, Stühler K, Wattenberg A, Meyer HE, Mahdavi J, Borén T, Ruhl S (2009) Identification of glycoprotein receptors within the human salivary proteome for the lectin-like BabA and SabA adhesins of Helicobacter pylori by fluorescence-based 2-D bacterial overlay. Proteomics 9: 1582-1592.

Bürgers R, Schneider-Brachert W, Reischl U, Behr A, Hiller K-A, Lehn N, Schmalz G, Ruhl S (2008) Helicobacter pylori in human oral cavity and stomach. Eur. J. Oral Sci. 116: 297-304.

Müller R, Ruhl S, Hiller K-A, Schmalz G, Schweikl H (2007) Adhesion of eukaryotic cells and Staphylococcus aureus to silicon model surfaces. J. Biomed. Mater. Res. A. 84A: 817-827.

Müller R, Gröger G, Hiller K-A, Schmalz G, Ruhl S (2007) Fluorescence-based bacterial overlay method for simultaneous in situ quantification of surface-attached bacteria. Appl. Environ. Microbiol. 73: 2653-2660.

Müller R, Hiller K-A, Schmalz G, Ruhl S (2006) Chemiluminescence-based detection and comparison of protein amounts adsorbed on differently modified silica surfaces. Anal. Biochem. 359: 194-202.

Aspholm M, Kalia A, Ruhl S, Schedin S, Arnqvist A, Sjöström R, Gerhard M, Semino-Mora C, Dubois A, Unemo M, Danielsson D, Teneberg S, Kon W, Berg D, Borén T (2006) Helicobacter pylori adhesion to carbohydrates. Meth. Enzymol. 417: 293-339.

Walz A, Wattenberg A, Meyer HE, Schmalz G, Blüggel M, Ruhl S (2006) Proteome analysis of glandular parotid and submandibular-sublingual saliva in comparison to whole human saliva by two-dimensional gel electrophoresis. Proteomics 6: 1631-1639.

Walz A, Odenbreit S, Mahdavi J, Borén T, Ruhl S (2005) Identification and characterization of binding properties of Helicobacter pylori by glycoconjugate arrays. Glycobiology 15: 700-708.

Ruhl S, Rayment SA, Schmalz G, Hiller K-A and Troxler RF (2005) Proteins in whole saliva during the first year of infancy. J. Dent. Res. 84: 29-34.

Ruhl S, Sandberg AL and Cisar JO (2004) Salivary receptors for the proline rich protein-binding and lectin-like adhesins of oral actinomyces and streptococci. J. Dent. Res. 83: 505-510.

Ruhl S, Hamberger S, Betz R, Sukkar T, Schmalz G, Seymour RA, Hiller K-A and Thomason JM (2004) Salivary proteins and cytokines in drug-induced gingival overgrowth. J. Dent. Res. 83: 322-326.

Takahashi Y, Ruhl S, Yoon J-W, Sandberg AL and Cisar JO (2002) Adhesion of viridans group streptococci to sialic acid-, galactose- and N-acetylgalactosamine-containing receptors. Oral Microbiol. Immunol. 17: 257-262.

Ruhl S, Cisar JO, Sandberg AL (2000) Identification of polymorphonuclear leukocyte and HL-60 cell receptors for adhesins of Streptococcus gordonii and Actinomyces naeslundii. Infect. Immun. 68: 6346-6354.

Takahashi Y, Sandberg AL, Ruhl S, Muller J, and Cisar JO (1997) A specific cell surface antigen of Streptococcus gordonii is associated with bacterial hemagglutination and adhesion to 2-3-linked sialic acid-containing receptors. Infect. Immun. 65: 5042-5051.

Ruhl S, Sandberg AL, Cole MF, and Cisar JO (1996) Recognition of IgA1 by oral actinomyces and streptococcal lectins. Infect. Immun. 64: 5421-5424.

Sandberg AL, Ruhl S, Joralmon RA, Brennan MJ, Sutphin MJ, and Cisar JO (1995) Putative glycoprotein and glycolipid polymorphonuclear leukocyte receptors for the Actinomyces naeslundii WVU45 lectin. Infect. Immun. 63: 2625-2631.