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 About Oral BiologyNew Home     July 27, 2016  

Ashu Sharma  PhD

Department of Oral Biology

Professor

Faculty Research Profile

 
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Education and Training:
  • PhD, University of Delhi, India (1988)
  • MS, Biochemistry/Endocrinology, University of Delhi, India (1983)
  • BS, Zoology, University of Delhi, India (1979)
Employment:
  • Professor, Oral Biology, University at Buffalo (2011-present)
  • Associate Professor, Oral Biology, University at Buffalo (2005-2011)
  • Assistant Professor (Tenure Track), Oral Biology, State University of New York at Buffalo (1999-2005)
  • Assistant Professor (Research), Oral Biology, State University of New York at Buffalo (1994-1998)
  • Post Doctoral Fellow, Roswell Park Cancer Institute (1988-1991)
Awards and Honors:
  • Advancement for the Dental Research Award, University at Buffalo (2012)
  • Principle Investigator Award, University at Buffalo (2005)
  • M.Sc. Prakash Memorial Gold Medal, University of Delhi, Delhi (1981)

Grants and Sponsored Research:
  • July 2014-July 2019
    T. forsythia TLR2 ligands and surface glycans coordinate periodontal inflammation
    NIH/NIDCR
    Role: Principal Investigator
    $1,250,000.00
  • May 2014-May 2016
    Novel mechanisms of peptidoglycan synthesis in T. forsythia
    NIH/NIDCR
    Role: Principal Investigator
    $325,000.00
  • September 2008-August 2012
    T helper cell responses in Tannerella forsythia-induced alveolar bone destruction
    NIH
    Role: Principal Investigator
    $1,000,000.00
  • April 2008-March 2013
    Tannerella forsythia interactions with host cells and other bacteria
    NIH
    Role: Principal Investigator
    $1,250,000.00
  • June 2006-December 2006
    Molecular genetic analysis of S. mutans cariogenicity
    NIH
    Role: Principal Investigator
  • June 2006-December 2006
    Oral Spirochetes-Molecular Genetic Analysis
    NIH
    Role: Principal Investigator
  • April 2003-April 2007
    Bacteroides forsythus BspA protein: Role in Virulence.
    NIH
    Role: Principal Investigator
    $700,000.00
  • April 2003-April 2007
    B. forsythus BspA protein: Role in Virulence
    NIH
    Role: Principal Investigator
    $175,000.00
  • August 1998-July 2000
    P. gingivalis fimbrillin expression in S. gordonii.
    Siga Pharmaceuticals, New York, NY
    Role: Principal Investigator
  • September 1997-September 2002
    Streptococcus gordonii as a vector for Porphyromonas gingivalis fimbrillin.
    NIH
    Role: Principal Investigator
    $527,542.00
  • December 1986-June 2006
    Molecular Aspects of PMN Function in Dental Disease
    NIH
    Role: Co-Investigator
    $180,000.00
See All (11 Total) >

Journal Articles:
See All (90 Total) >

Abstracts:
  • Rogers SC, Keinan D, Baier R, Mang T, Honma K, Sharma A. Examination of periodontal pocket biofilm growth and effects of PDT for perio-infection control. J Dent Res. 2013.
  • Hall L, Dunford R, Genco RJ, Sharma A. Humoral Immune Response to Tannerella Forsythia's Virulence Factor BspA. J Dent Res. 2010.
  • Mishima E, Sharma A. Molecular mechanisms of Tannerella forsythia invasion into epithelial cells. J Dent Res. 2010; 89.
  • Myeni Venkatasatya S, Settem P, Sojar HT, Sharma A. Functional epitopes of Tannerella forsythia BspA protein in TLR2 activation. J Dent Res. 2010; 89.
  • Sahingur SE, Alamgir S, Honma K, Sharma A, Schenkein HA. Periodontal bacterial DNA-mediated cytokine induction from human monocytic cells. J Dent Res. 2009; 88.
  • Onishi S, Honma K, Sharma A. TLR2-mediated epithelial cell activation by the Tannerella forsythia BspA protein. J Dent Res. 2008; 87.
  • Honma K, Perry MJ, Sharma A. Characterization of OxyR homologue of Tannerella forsythia. J Dent Res. 2008; 87.
  • Tanzer, JM, Thompson, AS, Sharma A, Vickerman MM, Scannapieco FA. Sucrose-independence of Streptococcus gordonii to compete with Streptococcus mutans. J Dent Res. 2008; 87.
  • Sharma A, Onishi, S, Inagaki, S, Honma, K. Toll-Like Receptor 2 Activation by Tannerella forsythia BspA Protein: Structure. Gordon Conference on Periodontal Disease, Italy. J Dent Res. 2006.
  • Inagaki S, Kuramitsu H, Sharma A. Contact-Dependent Regulation of Leucine-Rich Repeat BspA Protein of Tannerella forsythia. IADR/AADR/CADR 83rd General Session (March 9-12, 2005). J Dent Res. 2005.
  • Honma K, Inagaki S, Ishihara K, Okuda K, Kuramitsu H, Sharma A. Analyses of Tannerella forsythia Genes Involved in Biofilm Growth. IADR/AADR/CADR 83rd General Session (March 9-12, 2005). J Dent Res. 2005.
  • Onishi S, Inagaki S, Honma K, Sharma A. TLR2 Activation by Tannerella forsythia BspA Protein; Role of TLR1. IADR/AADR/CADR 83rd General Session (March 9-12, 2005). J Dent Res. 2005.
  • Sharma A, Kuramitsu H. Synergistic Oral Biofilm Formation by Periodontopathic Fusobacterium nucleatum and Bacteroides forsythus. American Society for Microbiology Conference on Biofilms. Victoria, British Columbia, Canada. 2003.
  • Ovrutsky D, Genco RJ, Sharma A. The Leucine-Rich Repeat Domain (LRR) of Bacteroides forsythus BspA Protein is Responsible for Activation on Monocyctic Cells. IADR Meeting, San Diego, CA. J Dent Res. 2002.
  • Sharma A, Neelamegham HT, Sojar H, Genco RJ. The Leucine-Rich Repeat Domain of Bacteroides forsythus (Tannerella forsythensis) BspA Protein Binds A- and B-Chains of Fibrinogen: Role in Platelet Function. IADR Meeting, San Diego, CA. J Dent Res. 2002.
  • Hajishengallis G, Sojar H, Sharma A, Martin M, DeNardin E, Genco RJ. TLR-4 Dependent Upregulation of Costimulatory Molecules in Macrophages by P. gingivalis FimA. IADR Meeting, San Diego, CA. J Dent Res. 2002.
  • Sojar H, Sharma A, Martin, M., Schifferle R, DeNardin E, Genco RJ. Host Regulatory Interactions Between P. gingivalis Fimbriae and LPS.. J Dent Res. 2001; 80SI(578).
  • Hajishengallis, G, Sojar H, Sharma A, Martin, M, Schifferle R, DeNardin E, Genco RJ. Host Regulatory Interactions Between P. gingivalis Fimbriae and LPS. IADR Meeting in Chiba, Japan. J Dent Res. 2001.
  • Gwinn M, Lewins MR, Sharma A, DeNardin E. Sequence Analysis of the Interleukin-8 (IL-8) Receptor in Localized Juvenile Periodontitis. J Dent Res. 1999; 78.
  • Sahingur SE, Gwinn MR, Sharma A, DeNardin E. Restriction Fragment Length Polymorphism in the Promoter Region of B-Fibrinogen Gene in Adult Periodontitis Patients and Healthy Subjects. J Dent Res. 1999; 78.
  • Sharma A, Sojar H, Novak EK, Swank RT, Kuramitsu H, Genco RJ. Identification of a Putative Gene Encoding Porphyromonas gingivalis Platelet-Aggregation Associated Protein (PgPAAP). IADR Meeting at Vacouver, Canada. J Dent Res. 1999.
  • Honma K, Sharma A, Sojar H, Genco RJ. Purification of Cell Surface Associated Protein of Bacteroides forsythus. J Dent Res. 1999; 78.
  • Ming G, Han Y, Sharma A, DeNardin E. Characterization of the Human IgG Fc Receptor of Fusobacterium nucleatum. IADR Meeting at Vancouver, Canada. J Dent Res. 1999; 78.
  • Sojar H, Han Y, Hamada N, Sharma A, DeNardin E. Role of the Amino Terminal Region of Porphyromonas gingivalis Fimbriae in Adherence to Epithelial Cells.. J Dent Res. 1999; 78.
  • Katancik JA, Sharma A, DeNardin E. Point Mutagenesis Studies of the Human Neutrophil CXC Chemokine Receptor-2. J Dent Res. 1999; 78.
  • Guo, M, Han, YP, Sharma A, DeNardin E. Characterization of the Human IgG Fc Receptors of Fusobacterium nucleatum. J Dent Res. 1999; 78.
  • Katancik, JA, Sharma A, DeNardin E. Point Mutagenesis Studies of the Human Neutrophil CXC Chemokine Receptor-2. J Dent Res. 1999; 78.
  • Gwinn, MR, Lewins, SN, Sharma A, DeNardin E. Sequence Analysis of the Interleukin-8 (IL-8) Receptor in Localized Juvenile Periodontitis. J Dent Res. 1999; 78.
  • Sahingur, SE, Gwinn, MR, Sharma A, DeNardin E. Restriction Fragment Length Polymorphisms in the Promoter Region of the B-Fibrinogen Gene in Adult Periodontitis Patients and Healthy Subjects. J Dent Res. 1999; 78.
  • Sharma A, Sojar H, Hruby DE, Kuramitsu H, Evans R, Genco RJ. Recombinants of Streptococcus gordonii Express Functional Domains of Porphyromonas gingivalis Fimbrillin. 76th IADR Meeting, Nice, France. J Dent Res. 1998; 77.
  • Sharma A, Novak, E, McGarry, M, Swank, RT, Kuramitsu H, Genco RJ. Characterization of Porphyromonas gingivalis-Induced Platelet Aggregation. ASM Conference on Microbes, Haemostasis and Vascular Biology, March 29 - April 1, 1998 at Gaveston, TX. 1998.
  • Gwinn M, Sharma A, DeNardin E. Sequence Analysis of Chemotactic Receptor DNA in Localized Juvenile Periodontitis. IADR Meeting in Nice, France. J Dent Res. 1998.
  • Katancik, JA, Sharma A, DeNardin E. Point Mutagenesis Studies of the Human Interleukin-8 Type B Receptor. AADR Minneapolis Meeting. J Dent Res. 1998.
  • Gwinn M, Sharma A, DeNardin E. Sequence Analysis of FMLP Receptor DNA in LJP. J Dent Res. 1998.
  • Sharma A, Hamada N, Sfintescu C, Nagata N, Sojar H, Kuramitsu H, Evans R, Genco RJ. Recombinant S. gordonii Expressing P. gingivalis Fimbrillin as Vectors for Immunization Against Periodontal Disease. AADR Meeting, Orlando, FL. J Dent Res. 1997.
  • Gwinn, M, Sharma A, DeNardin E. Single Strand Conformation Polymorphism Analysis of FMLP Receptor-Encoding DNA. J Dent Res. 1997; 76.
  • Gwinn, M, Sharma A, DeNardin E. Single Strand Conformation Polymorphism Analysis of the FMLP Receptor. J Dent Res. 1996; 75.
  • Gwinn, M, Sharma A, DeNardin E. Single Strand Conformation Polymorphism Analysis of FMLP Receptor DNA. 22nd Annual Maine Biological and Medical Sciences Symposium. The University of Maine at Orono. 1996.
  • Lala, A, Sharma A, Sojar H, Radel, SJ, Genco RJ, DeNardin E. Cloning and Expression of FMLP Receptor in E. coli. International Association for Dental Research, Chicago, IL. J Dent Res. 1993.
  • Amano A, Sojar H, Lee JY, Sharma A, Levine MJ, Genco RJ. Salivary components promote the attachment of p.gingivalis recombinant fimbrillin protein to hydroxyapatite.. J Dent Res. 1993.
See All (40 Total) >
Books and Book Chapters:
  • Genco RJ, Sojar H, Lee J-Y, Sharma A, Bedi G, Cho M, Dyer DW. Porphyromonas gingivalis Fimbriae: Structure, Function, and Insertional Inactivational Mutants. In: Molecular Pathogenesis of Periodontal Diseases. 1994.
  • Genco RJ, Sojar H, Lee, J-Y, Sharma A, Bedi, GS, Cho M, Dyer, DW. Porphyromonas gingivalis. Fimbriae: Structure, Function and Insertional Inactivation Mutants.. Molecular Pathogenesis of Periodontal Disease. 1994(2).
  • Allen HJ, Sharma A, Kisailus EC. Isolation of Galaptin, SDS-PAGE, Peptide Preparation and Binding Site Specificity.. Lectins in Cell Biology. 1900.
Professional Memberships:
  • Member, American Association for the Advancement of Science (AAAS) (2006)
  • Member of Advisory Group, Oralgen Data Base at the Los Alamos National Laboratories This is a consortium formed by the NIDCR (NIH) and the Los Alamos Labs to advise on genomic approaches for the study of oral bacteria and their role in pathogenesis. (2005)
  • Member, American Association for Dental Research (2000)
  • Member, American Society for Microbiology and AAAS (2000)
Presentations:
  • T. forsythia surface layer glycosylation and BspA modulate host immunity, First International conference on P. gingivalis and related bacterial species, Nagasaki University (2013)
  • Tannerella forsythia surface glycosylation: a strategy for manipulating host immunity, Oral Microbiome, Immunity & Chronic Disease, University at Buffalo (2013)
  • The role of Tannerella forsythia leucine-rich-repeat BspA protein in epithelial cell interactions, Gordon Conference on Periodontal Disease (2006)
  • Invited Speaker, Houston Medical Sciences Center, University of Texas at Houston (1999)
  • Novel oral streptococccal vaccine vectors (1999)
Service Activities:
  • School of Dental Medicine Promotion and Tenure Committee; Member (2014-2015)
  • School of Dental Medicine Graduate Committee, D.D.S./Ph.D., Oral Biology; Director (2010-2015)
  • School of Dental Medicine Student Research and Honors Committee; Member (2002-2015)
  • School of Dental Medicine Graduate Committee, Preliminary Exams, Oral Biology; Member (1999-2001)
  • School of Dental Medicine Graduate Committee, Thesis, Oral Biology; Member (1999-1999)
  • School of Dental Medicine Graduate Committee, D.D.S./Ph.D., Oral Biology; Member (1998-1998)
  • School of Dental Medicine Graduate Committee, Admissions, Oral Biology; Member

  • Judge; Poster Judging at UB Postdoc symposium (2015-2015)
  • Reviewer/multiple journals; Manuscript reviewing; Reviewer (2015-2015)
  • Ad Hoc Reviewer, Archives of Oral Biology; Ad Hoc Reviewer (2008)
  • Ad Hoc Reviewer, Journal of Clinical Periodontology; Ad Hoc Reviewer (2008)
  • Ad Hoc Reviewer, Gene; Ad Hoc Reviewer (2007)
  • Ad Hoc Reviewer, Journal of Thrombosis and Haemostasis; Ad Hoc Reviewer (2007)
  • Ad Hoc Reviewer, Odontology; Ad Hoc Reviewer (2007)
  • Journal Reviewer, FEMS Microbiology & Immunology; Reviewer (2006)
  • Journal Reviewer, Microbiology; Reviewer (2006)
  • Journal Reviewer, Vaccine Journal; Reviewer (2006)
  • Ad Hoc Reviewer, Journal of Periodontology; Ad Hoc Reviewer (2006)
  • IGBBS-member; recruit students for program; Member (2002-2005)
See All (19 Total) >

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Contact Information

Foster Hall
Room 311
3435 Main Street
Buffalo, NY 14214
Phone: (716) 829-2759
Fax: (716) 829-3942
Email: sharmaa@buffalo.edu


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Sharma, Ashu Ph.D Professor, Department of Oral Biology

Address:
311A Foster Hall
Buffalo, NY 14214
(716)829-2759
sharmaa@buffalo.edu

Research Profile

The human oral cavity is a highly diverse ecosytem containing more than 500 species of bacteria, including both cultivable and non-cultivable species. It is believed that existence of a select few Gram-negative anaerobes, known as the ‘red-complex’, is responsible for periodontitis or gum-associated disease. These bacteria live as biofilms and form bacterial plaque in spaces between gums and teeth. The bacterial products cause inflammation by inducing inflammtory cytokine release from host cells. Therefore, if these bacteria are not erradicated, inflammation continues and leads to destruction of tooth supporting tissue and eventually tooth loss. My lab studies the pathogenic mechanisms of the red-complex bacteria by utilizing molecular-genetic and biochemical approaches; the bacteria of the red-complex include Porphyromonas gingivalis, Tannerella forsythia (formerly Bacteroides forsythus) and Treponema denticola (a spirochete). Our overall objectives are to gain a better understanding of how these pathogenic bacteria initiate colonization, form biofilms and initiate tissue destructive host immune responses critical for disease progression. The research focuses on identifying virulence factors these bacteria produce and host-cell receptors involved in their recognition. Identification of these virulence factors and their receptor should pave the way for developing intervention strategies, such as vaccines, against periodontal bacteria. In this regard, we have developed genetic systems whereby non-pathogenic oral streptococci (Streptococci gordonii) can be engineered to expression vaccine antigens of choice. These genetically modified streptococcal vectors can be utilized for vaccine delivery by oral route.

Representative Publications Search Publications in MEDLINE

Book Chapters

A. Sharma, Genome functions of Tannerella forythia in bacterial communities. in: P.E. Kolenbrander, (Ed.), Oral Microbial Communities: Genome inquiry and interspecies communication, American Society for Microbiology, Washington, D. C., 2011.

Select Publications in peer reviewed journals (2006-2012)

1. Xie H, Hong J, Sharma A, Wang BY. Streptococcus cristatus ArcA interferes with Porphyromonas gingivalis pathogenicity in mice. J Periodontal Res. 2012 Mar 26. doi: 10.1111/j.16000765.2012.01469.x. [Epub ahead of print] PubMed PMID: 22448761
2. Stafford G, Roy S, Honma K, Sharma A. Sialic acid, periodontal pathogens and Tannerella forsythia: stick around and enjoy the feast! Mol Oral Microbiol. 2012 Feb;27(1):11-22. doi: 10.1111/j.2041-1014.2011.00630.x. Epub 2011 Nov 12. PubMed PMID: 22230462.
3. Roy S, Phansopa C, Stafford P, Honma K, Douglas CW, Sharma A, Stafford GP. Beta-hexosaminidase activity of the oral pathogen Tannerella forsythia influences biofilm formation on glycoprotein substrates. FEMS Immunol Med Microbiol. 2012 Jan 25. doi: 10.1111/j.1574-695X.2012.00933.x. [Epub ahead of print] PubMed PMID: 22276920
4. Roy S, Honma K, Douglas CW, Sharma A, Stafford GP. Role of sialidase in glycoprotein utilization by Tannerella forsythia. Microbiology. 2011 Nov;157(Pt 11):3195-202. Epub 2011 Sep 1. PubMed PMID: 21885482
5. Myneni SR, Settem RP, Connell TD, Keegan AD, Gaffen SL, Sharma A. TLR2 Signaling and Th2 Responses Drive Tannerella forsythia-Induced Periodontal Bone Loss. J Immunol. 2011 Jul 1;187(1):501-9. Epub 2011 Jun 1. PubMed PMID: 21632710; PubMed Central PMCID: PMC3119786.
6. Mishima E, Sharma A. Tannerella forsythia invasion in oral epithelial cells requires phosphoinositide-3-kinase activation and clathrin-mediated endocytosis. Microbiology. 2011 May 26. [Epub ahead of print] PubMed PMID: 21622527.
7. Hall LM, Dunford RG, Genco RJ, Sharma A. Levels of Serum IgG Specific to the BspA Protein of Tannerella forsythia are Related to the Periodontal Status. J Periodontol. 2011 May 24. [Epub ahead of print] PubMed PMID: 21609257.
8. Honma K, Mishima E, Sharma A. 2011. Role of Tannerella forsythia NanH Sialidase in Epithelial Cell Attachment. Infect Immun. 79:393-401. Epub 2010 Nov 15. PMID: 21078857
9. Sharma, A. Virulence mechanisms of Tannerella forsythia. Periodontology 2000. 54:106-116. 2010.
10. Sahingur SE, Xia XJ, Alamgir S, Honma K, Sharma A, and Schenkein HA. DNA from Porhyromonas gingivalis and Tannarella forsythia induce cytokine production from human monocytes. Molecular Oral Microbiology 25:123-135, 2010.
11. Loimaranta, V, Hytönen, J, Pulliainen, A. T, Sharma, A Jorma, J, Strömberg, N, and Finne, J. Leucine rich repeats of bacterial surface proteins serve as common pattern recognition motifs of human scavenger receptor Gp34. J. Biol. Chem, Epub ahead of print May 22, 2009.
12. Honma, K. Mishima, E., Ingaki, S. and Sharma A. The OxyR homologue in Tannarella forsythia regulates expression of oxidative stress responses and biofilm formation. Microbiology. 155:1912-1922 (Epub April 23). 2009.
13. Morozumi, T. Sharma, A and De Nardin E. The function al effects of the -455G/A polymorphism on the IL-6-induced expression of the b-fibrinogen gene may be due to linkage disequilibrium with other functional polymorphisms. Immunological Investigations 38: 1-13. 2009.
14. Onishi S, Honma K, Liang S, Stathopoulou P, Kinane D, Hajishengallis G, and *Sharma A. TLR2-mediated interleukin-8 expression in gingival epithelial cells by the Tannerella forsythia leucine-rich repeat BspA protein. Infect Immun. 76:198-205. 2008
15. Honma, K., Inagaki, S., Okuda, K., Kuramitsu, H. k., and *Sharma A. Role of a Tannerella forsythia Exopolysaccharide Synthesis Operon in Biofilm Development. Microbial Pathogenesis. 42:156-166. 2007.
16. Sahingur, S. E., Boehm, T. K., Sojar, H. T., Sharma, A. and De Nardin, E. Fibrinogen-neutrophil interactions in response to fMLP and Porphyromonas gingivalis fimbrial peptides. Immunol. Invest. 35:63-74. 2006.
17. Chen W, Honma K, Sharma A, Kuramitsu H. K. A universal stress protein of Porphyromonas gingivalis is involved in stress responses and biofilm formation. FEMS Microbiol Lett. 264:15-21. 2006
18. Inagaki, S., Onishi, S., Kuramitsu, H. K. and *Sharma, A. Porphyromonas gingivalis Vesicles Enhance Attachment and the Leucine-Rich Repeat BspA Protein is Required for Invasion of Epithelial Cells by Tannerella forsythia. Infect. Immun. 74: 5023-5028. 2006