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 About Oral BiologyNew Home     November 26, 2014  

Elaine M. Haase  PhD

Department of Oral Biology

Research Associate Professor

Specialty/Research Focus

Microbiology; Oral Biology

Faculty Research Profile

 
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Professional Summary:

I began my career as a general Medical Technologist and eventually specialized in clinical bacteriology in local hospital. I returned to school to earn an MS in Medical Technology specializing in Microbiology. I continued to work weekends in the hospital while working as a research technician in immunodermatology. I pursued further graduate work and earned a PhD degree in Microbiology from UB. During my thesis work I became interested in molecular mechanisms of infectious disease. As a post-doc I focused on characterizing the genes of the fimbrial operon and biofilm formation by the periodontopathogen Aggregatibacter actinomycetemcomitans. Currently I am working on gene regulation in A. actinomycetemcomitans by small non-coding sRNA. We have recently used RNAseq investigate the effect of amylase on Streptococcus gordonii and DNAseq to explore the phylogeny of amylase-binding proteins in the same bacteria.

Education and Training:
  • PhD, Microbiology, University at Buffalo (1995)
  • MS, Medical Technology, Microbiology Specialty, University at Buffalo (1984)
  • BS, Medical Technology, University at Buffalo (1975)
Employment:
  • Research Associate Professor, Oral Biology, State University of New York at Buffalo (2005-present)
  • Research Assistant Professor, Oral Biology, State University of New York at Buffalo (1998-2005)
  • Post Doctoral Fellow, Oral Biology, State University of New York at Buffalo (1994-1998)
  • Research Assistant, Oral Biology, State University of New York at Buffalo (1994)
  • Research Assistant, Microbiology and Immunology, State University of New York at Buffalo (1986-1994)
  • Research Technician, Microbiology and Immunology, State University of New York at Buffalo (1985-1986)
  • St. Mary‘s Hospital (1975-1991)
Awards and Honors:
  • Listed in Who's Who in America (2010)
  • American Society for Microbiology Student Travel Award (1994)
  • Listed in Who's Who Among Students in American Universities and Colleges (1992)
  • American Society for Microbiology Student Travel Award (1990)
  • Erwin Neter Award from WNY American Society of Microbiology (1990)

Research Expertise:
  • Microbiologist: Bacterial adhesion mechanisms, biofilm studies, protein analylsis, regulation of biofilms, and molecular biology.
  • Bioinformatics: Analysis of RNA-seq and DNA-seq data.
Grants and Sponsored Research:
  • April 2012-March 2017
    Amylase Binding and Gene Expression in S. gordonii.
    NIH-NIDCR
    Role: Co-Investigator
    $1,250,000.00
  • July 2004-June 2007
    Oral Health and Ventilator-Associated Pneumoniae
    NIH, USPHS
    Role: Co-Investigator
    $1,140,607.00
  • July 2004-January 2007
    New Salicylanilides to Treat Oral Diseases
    NIH/SBIR
    Role: Co-Investigator
  • April 2004-December 2008
    Amylase-Binding Streptococci, Dental Plaque and Caries
    NIH, USPHS
    Role: Co-Investigator
    $1,532,810.00
  • January 2002-December 2003
    Studies of the flp operon in A. actinomycetemcomitans
    National Institute for Dental & Craniofacial Research
    Role: Principal Investigator
    $100,000.00

Journal Articles:
  • Li Q, Pan C, Teng D, Lin L, Kou Y, Haase EM, Scannapieco FA, Pan Y. Porphyromonas gingivalis modulates Pseudomonas aeruginosa-induced apoptosis of respiratory epithelial cells through the STAT3 signaling pathway. Microbes Infect. 2014; 16(1).
  • Heo SM, Choi KS, Kazim LA, Reddy MS, Haase EM, Scannapieco FA, Ruhl S. Host Defense Proteins Derived from Human Saliva Bind to Staphylococcus aureus. Infect Immun. 2013; 81.
  • Nikitkova AE, Haase EM, Scannapieco FA. Taking the starch out of oral biofilm formation: molecular basis and functional significance of salivary a-amylase binding to oral streptococci. Appl Environ Microbiol. 2013; 79(2).
  • Violante TL, Haase EM, Vickerman MM. Collagen-binding streptococcal surface proteins influence the susceptibility of biofilm cells to endodontic antimicrobial solutions. J Endod. 2013; 39(3).
  • Amarasinghe JJ, Connell TD, Scannapieco FA, Haase EM. Novel iron-regulated and Fur-regulated small regulatory RNAs in Aggregatibacter actinomycetemcomitans. Mol Oral Microbiol. 2012; 27(5).
  • Nikitkova A, Haase EM, Scannapieco FA. Effect of starch and amylase on the expression of amylase-binding protein A in Streptococcus gordonii Mol Oral Microbiol. 2012.
  • Nikitkova AE, Haase EM, Vickerman MM, Gill SR, Scannapieco FA. Response of fatty acid synthesis genes to the binding of human salivary amylase by Streptococcus gordonii. Appl Environ Microbiol. 2012; 78(6).
  • Tanzer JM, Thompson A, Sharma K, Vickerman MM, Haase EM, Scannapieco FA. Streptococcus mutans out-competes Streptococcus gordonii in vivo. J Dent Res. 2012; 91(5).
  • Bank,TL, Dosen A, Giese RF, Haase EM, and Sojar HT. Atomic force spectroscopy evidence of non-specific adhesion of Aggregatibacter actinomycetemcomitans J Nanoscience Nanotechnol. 2011; 11.
  • Heo SM, Sung RS, Scannapieco FA, and Haase EM. Genetic Relationships Between Candida albicans Strains Isolated from Dental Plaque, Trachea, and Bronchoalveolar Lavage Fluid from Mechanically Ventilated Intensive Care Patients J Oral Microbiol. 2011; 3.
  • Vorrasi, J.B., Chaudhuri, B., Haase, E.M., and Scannapieco, F.A.. Identification and Characterization of Amylase-Binding Protein C from the Streptococcus mitis NS51 Mol Oral Microbiol. 2010.
  • Amarasinghe JJ, Scannapieco FA, Haase EM. Transcriptional and translational analysis of biofilm determinants of Aggregatibacter actinomycetemcomitans in response to environmental perturbation. Infect Immun. 2009; 77.
  • Pan Y, Teng D, Burke AC, Haase EM, Scannapieco FA. Oral Bacteria Modulate Invasion and Induction of Apoptosis in HEp-2 Cells by Pseudomonas aeruginosa. Microb Pathog. 2009; 46.
  • Chaudhuri B, Paju S, Haase EM, Vickerman MM, Tanzer JM, Scannapieco FA. Amylase-binding Protein B of Streptococcus gordonii is an Extracellular Dipeptidyl-peptidase. Infect Immun. 2008; 76(10).
  • Heo S-M, Haase EM, Lesse A, Gill SR, Scannapieco FA. Genetic Relationships Between Respiratory Pathogens Isolated from Dental Plaque and Bronchoalveolar Lavage Fluid from Mechanically Ventilated Intensive Care Patients. Clin Infect Dis. 2008.
  • Bernstein JM, Haase EM, Dryja D, Wolf J, Briles D, Wilding G. Bacterial Interference of Penicillin-Sensitive and Penicillin-Resistant Streptococcus pneumoniae by Streptococcus oralis in an Adenoid Organ Culture: Implications for the Treatment of Recurrent Upper Respiratory Tract Infections in Children and Adults. Ann Otol Rhinol Lyarngol. 2006; 115.
  • Haase EM, Scannapieco F, Bonstein T, Palmer RJ Jr. Environmental Influences on Actinobacillus actinomycetemcomitans Biofilm Formation. Arch Oral Biol. 2006; 51.
  • Haraokopakis E, Haase EM, Scannapieco F, Albzreh H, Hajishengallis G. Inhibition of Proinflammatory Activities of Major Periodontal Pathogens by Aqueous Extracts from Elder Flower (Sambucus nigra). J Periodontol. 2006; 77.
  • Haase EM, Stream JO, Scannapieco FA. Transcriptional Analysis of the 5‘-Terminus of the flp Fimbrial Gene Cluster from Actinobacillus actinomycetemcomitans. Microbiol. 2003; 149.
  • Paju Susanna, Bernstein JM, Haase EM, Scannapieco FA. Molecular Analysis of Bacterial Flora Associated with Chronically Inflamed Maxillary Sinuses. J Med Microbiol. 2003; 52.
  • Tanzer JM, Grant L, Thompson A, Li L, Rogers JD, Haase EM, Scannapieco FA. Amylase-Binding Protein A (AbpA) and B (AbpB) Differentially Affect Colonization of Rats‘ Teeth by Streptococcus gordonii. Microbiol. 2003; 149.
  • Tanzer JM, Baranowski LK, Scannapieco FA, Rogers JD, Haase EM. Oral Colonization and Cariogenicity of Streptococcus gordonii in Specific Pathogen-free TAN:SPFOM(OM)BR Rats Consuming Starch or Sucrose Diet. Arch Oral Biol. 2000; 46.
  • Brown AE, Rogers JD, Haase EM, Zelasko PM, Scannapieco FA. Prevalence of the Amylase-Binding Protein A Gene (abpA) in Oral Streptococci. J Clin Microbiol. 1999; 37.
  • Haase EM, Zmuda JL, Scannapieco FA. Identification and Molecular Analysis of Rough-Colony-Specific Outer Membrane Proteins of Actinobacillus actinomycetemcomitans. Infect Immun. 1999; 67.
  • Rogers JD, Haase EM, Brown AE, Douglas CWI, Gwynn JP, Scannapieco FA. Identification and Analysis of a Gene (abpA) Essential for Amylase Binding to Streptococcus gordonii. Microbiol. 1998; 144.
  • Haase EM, Yi K, Morse GD, Murphy TF. Mapping of Bactericidal Epitopes on the P2 Porin Protein of Nontypeable Haemophilus influenzae. Infect Immun. 1994; 62.
  • Haase EM, Campagnari AA, Sarwar J, Shero M, Wirth M, Cumming CU, Murphy TF. Strain-Specific and Immunodominant Surface Epitopes of the P2 Porin Protein of Nontypeable Haemophilus influenzae. Infect Immun. 1991; 59.
  • Klingman KL, Jansen(Haase) EM, Murphy TF. Nearest Neighbor Analysis of Outer Membrane Proteins of Nontypeable Haemophilus influenzae. Infect Immun. 1988; 56.
  • Haase EM, Deng JS, Beutner EH, Kumar V, Chorzelski TP. Comparison of Commercial Kits for the Detection of Anti-nDNA Antibodies Using Crithidia luciliae. Amer J Clin Path. 1987; 87.
See All (29 Total) >

Abstracts:
  • Stuart, W., Haase, E., and Scannapieco, F.A.. Leukotoxin secretion and beta-hemolysis in Aggregatibacter actinomycetemcomitans. Dental Student Research Day. 2013.
  • Reardon, J., Haase, E.M., and Scannapieco, F.A.. AbpA as a potential salivary amylase inhibitor. Dental Student Research Day. 2013.
  • Maddi, A, Haase EM, Scannapieco FA. Proteomic analysis of amylase and of an amylase-binding-protein A interactive components in Streptococcus gordonii. IADR 91st Meeting. 2013.
  • Feng, X, Haase EM, Scannapieco FA. The transcriptional changes of Streptococcus gordonii amylase-binding protein A (abpA-) mutant in TSBY medium. 50th Anniversary Oral Biology Symposium. 2013.
  • Kuo, Y, Haase EM, Scannapieco FA. Identification and protein sequencing of amylase-binding proteins from oral streptococcal species. 50th Anniversary Oral Biology Symposium. 2013.
  • Haase EM, Amarasinghe JA, Scannapieco FA. Novel Fur and iron-regulated small regulatory RNAs of Aggregatibacter actinomycetemcomitans. J Dent Res. 2012.
  • Haase EM. Starch and salivary alpha-amylase induce expression of Streptococcus gordonii AbpA. J Dent Res. 2012.
  • Violante T, Haase EM, Cianco SG, and Vickerman MM. Effects of antiseptics on Streptococcus gordonii biofilms. J Dent Res. 2012.
  • Hei S, Choi K, Kazim LA, Haase EM, Reddy M, Scannapieco FA, and Ruhl SH. Binding between human salivary components and Staphylococcus aureus. J Dent Res. 2012.
  • Nikitkova, A., Haase, E.M., Gill, S.R., Vickerman, M.M., and Scannapieco, F.A.. Roles of salivary amylase and AbpA in Streptococcus gordonii gene expression and host adaptation. ASM 111th General Meeting. 2011.
  • Heo, S-M., Choi, K.S., Kazam, A.L., Reddy, M.S., Haase, E.M., Scannapieco, F.A., and Ruhl, S.H.. Characterization of human salivary components associated with Staphylococcus aureus. J Dent Res. 2010.
  • Amarasinghe, JJ, Scannapieco, FA and Haase, EM. Characterization of Novel Fur- and Iron-regulated sRNA Regulatory Mechanisms Involved in Biofilm Formation of Aggregatibacter actinomycetemcomitans. ASM 109th General Meeting. 2009.
  • Dosen J, Bank TL, Haase EM, Sojar H, Geise RF. Adhesion of Aggregatibacter actinomycetemcomitans (Aa) Flp1 fimbriae to surfaces with different thermodynamic properties. 2nd Annual AFM BioMed Conference. 2008.
  • Amarasinghe JJ, Scannapieco FA, Haase EM. Novel Iron-Regulated sRNAs Involved in Biofilm Formation of Aggregatibacter actinomycetemcomitans.. J Dent Res. 2008; 87.
  • Heo S-M, Haase EM, Scannapieco FA. Genetic Relationship Between Respiratory Pathogens Isolated from the Mouth and Lung.. J Dent Res. 2008; 87.
  • Haase EM, Dunn J, Evans R, Scannapieco FA. Evaluation of Antimicrobial Efficacy Against Biofilms Using the Calgary Biofilm Device.. 4th ASM Conference on Biofilms. 2007.
  • Heo S-M, Haase EM, Scannapieco F. Molecular Epidemiology of Plaque and Lung Bacteria from Ventilated Patients.. J Dent Res. 2007; 86.
  • Amarasinghe JJ, Haase EM, Scannapieco FA. Comparison of Transcription and Translation of Biofilm Determinants of Aggregatibacter actinomycetemcomitans in Response to Environmental Changes. ASM 107th General Meeting. 2007.
  • Amarasinghe JJ, Haase EM, Scannapieco FA. Environmental influences on biofilm determinant expression in Actinobacillus actinomycetemcomitans.. ASM 106th General Meeting. 2006.
  • Haase EM, Bonstein T, Palmer RJ Jr, Scannapieco FA. Heterogeneity of Biofilm Formation Among Strains of Actinobacillus actinomycetemcomitans.. ASM 105th General Meeting. 2005.
  • Haase EM, Legler R, Bonstein T, Bush PJ, Scannapieco FA. An in vitro model assessing tooth whitening agent effectiveness.. J Dent Res. 2004; 83.
  • Haase EM, Collins JM, Wang Y, Chen C, Scannapieco FA. Characterization of selected mutants of the flp operon of Actinobacillus actinomycetemcomitans.. J Dent Res. 2003; 82.
  • Paju S, Haase EM, Tanzer JM, Scannapieco FA. Prevalence of the Gene Encoding Amylase-Binding Protein B, a Putative Peptidase, in Oral Streptococci.. J Dent Res. 2003; 82.
  • Haase EM, Palmer RJ Jr, Wang Y, Chen C, Kolenbrander P, Scannapieco FA. Localization of the rough colony protein A (RcpA) of Actinobacillus actinomycetemcomitans by immunofluorescence.. J Dent Res. 2002; 81.
  • Paju S, Bernstein JM, Haase EM, Scannapieco FA. Non-Cultivable Oral Bacterial Flora of Chronically Inflamed Maxillary Sinuses.. J Dent Res. 2002; 81.
  • Haase EM, Stream JO, Scannapieco FA. Transcriptional Analysis of the 5‘-Terminus of the flp Fimbrial Gene Cluster of Actinobacillus actinomycetemcomitans.. ASM 101st General Meeting. 2001.
  • Haase EM, Scannapieco FA. RNA Analysis of Rough-Colony-Specific Outer Membrane Proteins of Actinobacillus actinomycetemcomitans.. J Dent Res. 2000; 79.
  • Haase EM, Zmuda JL, Scannapieco FA. Identification of Rough-Colony-Phenotype Specific Proteins of Actinobacillus actinomycetemcomitans.. J Dent Res. 1999; 78.
  • Tanzer JM, Baranowski LK, Scannapieco FA, Rogers J, Haase EM. Streptococcus gordonii Colonizes TAN:SPFOM(OM)BR Rats and is Cariogenic with Sucrose.. J Dent Res. 1998; 77.
  • Sanjun IS, Steinhorn DM, Haase EM, Scannapieco FA. Perfluorocarbons Decrease Bacterial Adhesion to Epithelial Cells.. Ped Res. 1998; 43(S2).
  • Haase EM, Rogers JD, Scannapieco FA. Protein Characterization of an Amylase-Binding Deficient Mutant of Streptococcus gordonii.. ASM 97th General Meeting. 1997.
  • Haase EM, Scannapieco FA. Molecular Cloning of a Putative Amylase-Binding Component from Streptococcus gordonii G9B.. J Dent Res. 1996; 75.
  • Rogers JD, Cranston B, Haase EM, Scannapieco FA. Characterization of an Amylase-Binding Deficient Mutant of Streptococcus gordonii Challis.. J Dent Res. 1996; 75.
  • Haase EM, Yi KC, Morse GD, Murphy TF. Mapping of Bactericidal Epitopes on the P2 Porin of Nontypeable Haemophilus influenzae.. ASM 94th General Meeting. 1994.
  • Haase EM, Murphy TF. Monoclonal Antibody Analysis of P2, the Major Outer Membrane Protein of Nontypeable Haemophilus influenzae.. ASM 90th General Meeting. 1990.
  • Klingman KL, Haase EM, Murphy TF. Nearest Neighbor Analysis of Outer Membrane Protein of Nontypeable Haemophilus influenzae.. ASM 88th General Meeting. 1988.
See All (36 Total) >
Professional Memberships:
  • American Dental Education Association Professional Membership (2006)
  • International/American Association for Dental Research Professional Membership. (1996)
  • American Society for Microbiology Professional Membership (1988)
  • American Society for Clinical Pathologists (1975-2001)
Presentations:
  • Transcriptional changes of Streptococcus gordonii amylase-binding protein (abpA) mutant in TSBY medium, Gordon Conference, Salve Regina University (2013)
  • Studies of the Flp Locus of Actinobacillus actinomycetemcomitans, Seminar Series, University of Southern California (2003)
Service Activities:

  • St. Christopher Church, Religious Education; Office assistant for religious education classes. Assist in attendance, answering phone, and other needed tasks. Volunteer at annual Summerfest celebration.; Volunteer (2002)
  • Western New York American Society for Microbiology; Current member of the WNY ASM executive committee. Served as Secretary-Treasurer for 3 years, president-elect 1 year, president 1 year, past president for 3 years.; Committee Member (2000)
  • Laboratory Assistant; Laboratory Assistant for the summer program for Ernest Witebsky Center for Immunology.; Volunteer (1988-1988)

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Contact Information

Foster Hall
Room 109B
3435 Main Street
Buffalo, NY 14214
Phone: (716) 829-2520
Fax: (716) 829-3942
Email: haase@buffalo.edu


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Haase, Elaine Ph.D. Research Associate Professor, Department of Oral Biology

Address:
109b Foster Hall
Buffalo, NY 14214
(716)829-2520
haase@buffalo.edu

Research Profile

Biofilms are communities of bacteria attached to a surface enclosed in a matrix of extracellular polysaccharides (EPS), DNA dissolved nutrients and other biological molecules. Biofilm-associated organisms cause up to 80% of all infections. Dental plaque is a typical multispecies biofilm. The focus of our research is to define the molecular basis of biofilm formation by the periodontopathogen Aggregatibacter (Actinobacillus) actinomycetemcomitans. This organism is associated with localized aggressive periodontitis, a destructive form of periodontitis occurring primarily in adolescents, some forms of refractory periodontitis, and is a causative agent of a variety of extra-oral infections including endocarditis abscesses and septicemia. We have shown that biofilm varies quantitatively in response to environmental conditions commonly found in the oral cavity, including variable pH, nutrient availability, oxygen tension and iron. Primary biofilm determinants include fimbriae and lipopolysaccharide (LPS) involved in adhesion, along with EPS that serves in intercellular aggregation and as a protective barrier for the cells of the biofilm. We are investigating the role of small non-coding RNA in the post-transcriptional regulation of biofilm determinant genes (fimbriae, LPS and EPS) in response to iron-limited conditions. Through the use of molecular biology techniques, protein analysis, bioinformatics, and microarrays we aim to identify the pathways involved in the maintenance of biofilm by these sRNAs. We also have used the Calgary device and other microtiter systems to evaluate the antimicrobial efficacy of a new therapeutic agent against monospecies biofilms of oral microorganisms.

Representative Publications Search Publications in MEDLINE

1. Amarasinghe, J.J., E.M. Haase, and F.A Scannapieco. Involvement of iron-regulated non-coding small RNA molecules in biofilm formation of Aggregatibacter actinomycetemcomitans. (manuscript in preparation).
2. Amarasinghe, J.J., E.M. Haase, and F.A. Scannapieco. Comparison of transcription and translation of biofilm determinants of Aggregatibacter actinomycetemcomitans in response to environmental changes. (manuscript in preparation).
3. Haase, E.M., T. Bonstein, R.J. Palmer Jr., and F.A. Scannapieco. Environmental influences on Actinobacillus actinomycetemcomitans biofilm formation. Archiv. Oral Biol., 51:299-314, 2006.
4. Haase, E.M., J.O. Stream, and F.A. Scannapieco. Transcriptional analysis of the 5’-terminus of the flp fimbrial gene cluster from Actinobacillus actinomycetemcomitans. Microbiol. 149:205-215, 2003.
5. Haase, E.M., J.L. Zmuda, and F.A. Scannapieco. Identification and molecular analysis of rough-colony-specific outer membrane proteins of Actinobacillus actinomycetemcomitans. Infect. Immun. 67:2901-2908, 1999.
6. Haase, E.M., J.A. Dunn, R.T. Evans, and F.A. Scannapieco. Evaluation of antimicrobial efficacy of a new therapeutic agent against oral biofilms using the Calgary biofilm device. (manuscript submitted).